Metabolomic Profiling of Seasonal Katsumadin Production in Ternstroemia lineata
Por:
Soto-Díaz, AU, Villarreal, ML, Medrano, NS, Victorio-De los Santos, M, Sciutto, E, Cerón, JAE, Schripsema, J, Cardoso-Taketa, AT
Publicada:
13 mar 2026
Resumen:
Metabolomic profiling of the leaves, floral buds, flowers, and fruits of Ternstroemia lineata was conducted across the dry and rainy seasons in Mexico. The presence of katsumadin was determined and its antibacterial activity was evaluated. Multivariate data analysis was performed using proton nuclear magnetic resonance (H-1 NMR) and Principal Component Analysis (PCA). Katsumadin was identified by H-1 NMR and, quantified by HPLC, and its antibacterial activity was assessed using a microdilution assay. The results revealed conserved H-1 NMR signals among leaf, flower buds, flowers and fruits, as well as signals that were either organ-specific or time-dependent. The spatial and temporal distribution of katsumadin was monitored over a nine-month period. During the dry season, katsumadin reached 1.5 mg/g DW in the leaves, whereas it was not detected during the rainy season. Higher katsumadin contents were observed in floral buds, fruits, and flowers (6.09 +/- 0.10, 3.0 +/- 0.79, and 3.4 +/- 0.42 mg/g DW, respectively). In addition, katsumadin was evaluated against Salmonella Typhi and Pseudomonas aeruginosa, exhibiting a minimum inhibitory concentration and bactericidal effect at 0.5 mg/mL.
Filiaciones:
Univ Autonoma Estado Morelos, Ctr Invest Biotecnol, Cuernavaca 62210, Mexico
Univ Autonoma Estado Morelos, Fac Ciencias Biol, Cuernavaca 62210, Mexico
Univ Autonoma Nayarit, Unidad Acad Ciencias Quim Biol & Farmaceut, Nayarit 63000, Mexico
Univ Nacl Autonoma Mexico, Dept Inmunol, Inst Invest Biomed, Ciudad De Mexico 04510, Mexico
Univ Estadual Norte Fluminense, Grp Metabol, BR-28013602 Campos Dos Goytacazes, RJ, Brazil
Green Submitted, gold
|