Valorization of tequila agave leaves for succinic acid production by Actinobacillus succinogenes
Por:
Flores-Méndez D.A., Guatemala-Morales G.M., Vallejo-Giraldo A., Toriz G., Gosset G., Corona-González R.I.
Publicada:
1 ene 2026
Resumen:
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2026.Tequila agave leaves (TAL) are residues from the tequila industry left in the fields. They pose a serious environmental threat, as a source of stenches and pest infestations, since approximately 700,000 tons are generated annually. This study proposes the integral use of TAL to produce succinic acid, since TAL contain juice, rich in soluble carbohydrates, and fibers that can be hydrolyzed. The juice was extracted from the fibers and its chemical composition was determined. Juice, fibers and delignified fibers were subjected to different hydrolysis treatments to obtain fermentable sugars: acid (H2SO4 or HCl), enzymatic (MacerexR or CeluzymeR), and combined acid-enzymatic. The treatments that resulted in a higher sugar concentration were selected for succinic acid production with Actinobacillus succinogenes. It was found that the juice had a sugar content (glucose and fructose) of 31.3 g/L. The fibers, on the other hand, were composed primarily of cellulose and hemicellulose (70 wt%) and the highest concentration of fermentable sugars (28.4 g/L; 42% conversion efficiency) was achieved with fibers HCl-Celuzyme hydrolysis. Fermentations with A. succinogenes were carried out using the raw juice and fiber hydrolyzates (acid, enzymatic, and HCl-Celuzyme). The highest production of succinic acid was obtained with the juice (12.5 g/L succinic acid, YS/M = 0.48 g/g, rS = 0.70 g/L·h) and with HCl – Celuzyme hydrolyzates (13.6 g/L succinic acid, YS/M = 0.57 g/g, rS = 0.60 g/L·h). This study demonstrates that the leaves from tequila agave represent a promising renewable source to obtain substrates to produce succinic acid by A. succinogenes.
Filiaciones:
Flores-Méndez D.A.:
Departamento de Ingeniería Química, Tecnológico Nacional de México en Celaya, Antonio García Cubas #600, 38010, Celaya, Guanajuato, México
Guatemala-Morales G.M.:
Tecnología Alimentaria, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco, A.C. (CIATEJ), A.C. Av. Normalistas 800, Colinas de la Normal, C.P. 44270, Guadalajara, Jalisco, México
Vallejo-Giraldo A.:
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Toriz G.:
Departamento de Madera Celulosa y Papel, Universidad de Guadalajara, Km. 15.5 carretera Guadalajara-Nogales, C.P. 45110, Zapopan, Jalisco, México
Gosset G.:
Departamento de Ingeniería Celular y Biocatálisis, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
Corona-González R.I.:
Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blv. M. García Barragán 1421, C.P. 44430, Guadalajara, Jalisco, México
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