Pharmacokinetic Profile of Extracts from the Chayote (Sechium edule) H387 07 Hybrid and Phytochemical Characterization of Its Segregant H387 M16 for Potential Therapeutic Applications
Por:
Delgado-Tiburcio, EE, Soto-Hernández, RM, Aguiñiga-Sánchez, I, Cadena-Iñiguez, J, Ruiz-Posadas, LD, Peña-Valdivia, CB, Gómez-Yáñez, H
Publicada:
1 oct 2025
Resumen:
The hybrid Sechium edule H387 07, commonly known as chayote, has shown potential as an antiproliferative, cytotoxic, and pro-apoptotic agent in the murine leukemia cell lines P388 (macrophagic) and J774 (monocytic) and in the myelomonocytic leukemia cell line WEHI-3. However, despite these reported bioactivities, its pharmacokinetic profile remains largely unexplored. Understanding the absorption, distribution, and elimination of this hybrid is critical for addressing unmet therapeutic needs and for advancing the development of natural product-based therapies. These effects are attributed to the presence of phenols, flavonoids, and cucurbitacins in its organic extracts. In this study, the pharmacokinetic parameters of secondary metabolites from methanolic extracts of Sechium H387 07 were evaluated after oral administration in mice, while its segregant H387 M16 was subjected to complementary phytochemical characterization. Methanolic extracts of Sechium edule H387 07 were orally administered to mice at doses of 8, 125, and 250 mg/kg, and plasma, liver, and urine samples were collected at 1, 6, 24, and 48 h post-treatment. High-performance liquid chromatography (HPLC) identified polyphenols and cucurbitacins, notably cucurbitacin B (CuB) and cucurbitacin IIA (CuIIA), in the biological samples, and pharmacokinetic variables such as the maximum plasma concentration (Cmax), time to reach maximum concentration (Tmax), half-life (T1/2), and volume of distribution (Vd) were determined. For instance, CuB exhibited a Cmax of 37.56 mu g/mL at 1 h post-dose after oral administration of 125 mg/kg, confirming its rapid absorption and systemic distribution. Notably, the presence of CuIIA in plasma was documented for the first time, along with the pharmacokinetic profiles of apigenin, phloretin, CuB, CuE, and CuI. In parallel, the segregant H387 M16 was characterized via colorimetric assays, thin-layer chromatography (TLC), HPLC, and antioxidant activity tests, which revealed high levels of flavonoids, phenols, and cucurbitacins, with an antioxidant activity of approximately 75% at the highest tested dose (1 mg/mL), supporting its suitability for future bioassays. Overall, these findings not only provide novel pharmacokinetic data for key metabolites of the H387 07 hybrid but also establish the phytochemical and antioxidant profile of its segregant H387 M16. This dual characterization strengthens the evidence of the therapeutic potential of Sechium genotypes and provides a valuable foundation for future studies aiming to develop standardized protocols and explore translational applications in drug development and natural product-based therapies.
Filiaciones:
Delgado-Tiburcio, EE:
Postgrad Coll Campus Montecillo, Bot Dept, Km 36-5 Carretera Mexico Texcoco, Texcoco 56230, Mexico
Soto-Hernández, RM:
Postgrad Coll Campus Montecillo, Bot Dept, Km 36-5 Carretera Mexico Texcoco, Texcoco 56230, Mexico
Aguiñiga-Sánchez, I:
Univ Nacl Autonoma Mexico, FES Zaragoza, Res Unit Cell Differentiat & Canc, Hematopoiesis & Leukemia Lab, Mexico City 09230, Mexico
Cadena-Iñiguez, J:
Postgrad Coll, Innovat Nat Resource Management, Campus San Luis Potosi, Salinas De Hidalgo 78622, San Luis Potosi, Mexico
Ruiz-Posadas, LD:
Postgrad Coll Campus Montecillo, Bot Dept, Km 36-5 Carretera Mexico Texcoco, Texcoco 56230, Mexico
Peña-Valdivia, CB:
Postgrad Coll Campus Montecillo, Bot Dept, Km 36-5 Carretera Mexico Texcoco, Texcoco 56230, Mexico
Gómez-Yáñez, H:
Postgrad Coll Campus Montecillo, Bot Dept, Km 36-5 Carretera Mexico Texcoco, Texcoco 56230, Mexico
Green Submitted, gold, All Open Access; Gold Open Access; Green Open Access
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