Titers of IgG, IgM, and IgA Against SARS-CoV-2 in Healthcare Workers from a General Hospital in Mexico City
Por:
González-Arenas, NR, Chavez-Vargas, MD, Prado-Calleros, H, Ramirez-Hinojosa, JP, Martinez-Hernandez, F, Olivo-Diaz, A, Maravilla, P, Romero-Valdovinos, M, Avila-Ramirez, G
Publicada:
22 ago 2025
Resumen:
Objectives: The aim of the present study was to better understand the antibody concentrations in healthcare workers (HCWs) from a hospital in Mexico City with a high density of COVID-19 patients. Methods: Up to 243 HCWs were recruited in 2020 and 2022 and were sorted into three groups: hybrid immunity (HI, natural infection plus vaccination), vaccine-induced immunity (VI), and unvaccinated but RT-qPCR negative at the beginning of the pandemic (UV). Peripheral blood and nasopharyngeal swab samples were obtained; additionally, saliva samples were obtained from the UV group. The titers of IgG, IgM, and IgA against the SARS-CoV-2 receptor-binding domain (RBD) and nucleocapsid (NCP) proteins were assessed using an in-house ELISA, and positivity to the virus was determined via RT-qPCR. Results: Most HI and VI participants were positive for serum anti-RBD IgG (92.8% and 100%, respectively), while 26.6% (for HI) and 19% (for VI) were positive for anti-NCP IgG. Regarding serum anti-RBD IgA, the VI and HI groups had positive rates of 87.3% and 66%, respectively. In contrast, the UV group showed a rate of 5.7% but the positivity for IgA in saliva was higher (52% for RBD and 35% for NCP). In addition, the highest antibody titers were obtained for anti-RBD IgG and IgA in the HI and VI groups, respectively. In saliva, the IgA antibody titer was higher for the RBD antigen (1:1280). Conclusions: These results strengthen our understanding of antibody concentrations in HCWs during two critical years of the pandemic in a general hospital with many COVID-19 patients.
Filiaciones:
González-Arenas, NR:
Hosp Gen Dr Manuel Gea Gonzalez, SSA Calzada Tlalpan 4800,Col Secc 16, Mexico City 14080, Mexico
Chavez-Vargas, MD:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, Mexico
Prado-Calleros, H:
Hosp Gen Dr Manuel Gea Gonzalez, SSA Calzada Tlalpan 4800,Col Secc 16, Mexico City 14080, Mexico
Ramirez-Hinojosa, JP:
Hosp Gen Dr Manuel Gea Gonzalez, SSA Calzada Tlalpan 4800,Col Secc 16, Mexico City 14080, Mexico
Martinez-Hernandez, F:
Hosp Gen Dr Manuel Gea Gonzalez, SSA Calzada Tlalpan 4800,Col Secc 16, Mexico City 14080, Mexico
Olivo-Diaz, A:
Hosp Gen Dr Manuel Gea Gonzalez, SSA Calzada Tlalpan 4800,Col Secc 16, Mexico City 14080, Mexico
Maravilla, P:
Hosp Gen Dr Manuel Gea Gonzalez, SSA Calzada Tlalpan 4800,Col Secc 16, Mexico City 14080, Mexico
Romero-Valdovinos, M:
Hosp Gen Dr Manuel Gea Gonzalez, SSA Calzada Tlalpan 4800,Col Secc 16, Mexico City 14080, Mexico
Avila-Ramirez, G:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Mexico City 04510, Mexico
Green Submitted, gold, All Open Access; Gold Open Access; Green Accepted Open Access; Green Open Access
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