Characterization of human mesenchymal stem cell according to delivery route and its correlation with clinical-gynecological history
Por:
Martínez-Castillo, M, Altamirano-Mendoza, I, León-Mancilla, B, Santana-Vargas, D, García-Cervantes, E, Guerrero-Bustos, R, Gutiérrez-Reyes, G
Publicada:
1 jul 2025
Resumen:
Objective: To assess the pluripotency profile of human mesenchymal stem cells (hMSCs) as influenced by delivery mode and obstetric-gynecological background. Methods: Thirty-nine placentas were included. A fragment of the AM (5 cm) was utilized to obtain the AM-hMSCs. The cell cultures were monitored until 90% confluence, before carrying out expansion (passages). The cultures, cell morphology, adhesion, immunophenotyping characterization, and differentiation capacity were evaluated and compared with hMSC from bone marrow (BM-hMSC). T-student and Mann-Whitney U tests were carried out. Statistical significance was set at a p < 0.05. Results: No differences were observed in the proliferation and expansion of AM-hMSC obtained by vaginal or cesarean delivery route. Similar results were observed in the immunological characterization; however, CD105 was significantly lower compared with BM-hMSC. Nevertheless, cells from vaginal or cesarean delivery route showed great differentiation capacity to adipogenic, chondrogenic, and osteogenic lineages. Conclusions: The delivery route, clinical data, and obstetric and gynecologic histories are no limitations for using the AM to obtain hMSC and its possible application in Regenerative Medicine.
Filiaciones:
Martínez-Castillo, M:
Univ Nacl Autonoma MexicoUNAM, Fac Med, Expt Med Res Unit, Ciudad De Mexico, Mexico
Altamirano-Mendoza, I:
Univ Nacl Autonoma MexicoUNAM, Fac Med, Expt Med Res Unit, Ciudad De Mexico, Mexico
Inst Nacl Perinatol Isidro Espinosa los Reyes, Res Directorate, Ciudad De Mexico, Mexico
León-Mancilla, B:
Univ Nacl Autonoma MexicoUNAM, Fac Med, Expt Med Res Unit, Ciudad De Mexico, Mexico
UNAM, Fac Med, Dept Surg, Ciudad De Mexico, Mexico
Santana-Vargas, D:
Univ Nacl Autonoma MexicoUNAM, Fac Med, Expt Med Res Unit, Ciudad De Mexico, Mexico
García-Cervantes, E:
Hosp Gen Mexico Dr Eduardo Liceaga, Dept Gynecol & Obstet, Ciudad De Mexico, Mexico
Guerrero-Bustos, R:
Hosp Gen Mexico Dr Eduardo Liceaga, Dept Gynecol & Obstet, Ciudad De Mexico, Mexico
Gutiérrez-Reyes, G:
Univ Nacl Autonoma MexicoUNAM, Fac Med, Expt Med Res Unit, Ciudad De Mexico, Mexico
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