Optimal Mechanical Properties and High Cellular Proliferation on Hybrid PCL/HaP/Col/SA Scaffolds for Tissue Engineering
Por:
González-Alva, P, Gómez-Lizárraga, KK, González-Parra, R, Barba, A, Bañuelos, JG, Hernández, M
Publicada:
1 ago 2025
Ahead of Print:
1 jun 2025
Resumen:
This study determines the mechanical properties, surface features, and the cellular response of mesenchymal cells of an electrospun scaffold of polycaprolactone (PCL) combined with hydroxyapatite (HaP), collagen (Col), and sodium alginate/poly (vinyl alcohol) (SA/PVA) for applications in tissue engineering to restore injured tissue. Four different HaP structures were separately incorporated into the different scaffolds: (1) nanometric HaP, (2) micrometric mu HaP, (3) functionalized nanometric HaPf, and (4) natural-decellularized bovine condyle to produce a HaP-like compound NKB. The morphological characterization was carried out with scanning electron microscopy, atomic force microscopy, and contact angle measurements. This indicated a heterogeneous cross-linked scaffold with porosity and rounded beads, which varied depending on the HaP used. The NKB scaffold developed the highest roughness profile (2304.3 nm) and contact angle (121.6 degrees), inducing an optimal anchoring profile for the cellular culture. The mechanical properties assessed with the tensile test demonstrated a more fragile condition after the cross-linked process. The cell viability and proliferation properties were evaluated with a resazurin assay and fluorescent staining with Calcein AM and counterstained with DAPI. The initial adhesion of hFoB 1.19 cells was similar in all conditions. Nanosize HaP supported cell growth, especially at 168 h, with the higher density of cells observed in the NKB scaffold. Also, more cell-cell contact formation was observed in the nanosized compounds, with larger areas covered by clusters, sheets, or roads of cells. The mu Hap scaffold supported cell growth but appeared to be less attractive to osteoblast cells and demonstrated lower adhesion and less covered area. The NKB scaffold enhanced the cell activity, cell adhesion, proliferation, and cell-cell interactions.
Filiaciones:
González-Alva, P:
Univ Nacl Autonoma Mexico, Fac Odontol, Lab Bioingn Tejidos, Mexico City, Mexico
Gómez-Lizárraga, KK:
Univ Nacl Autonoma Mexico, CONAHCyT Fellow Inst Invest Mat, Mexico City, Mexico
González-Parra, R:
Univ Nacl Autonoma Mexico, Fac Ingn, Div Ingn Mecan & Ind DIMEI, Mexico City, Mexico
Barba, A:
Univ Nacl Autonoma Mexico, Fac Ingn, Div Ingn Mecan & Ind DIMEI, Mexico City, Mexico
Bañuelos, JG:
Univ Nacl Autonoma Mexico, LUCE, Inst Ciencias Aplicadas & Tecnol, Mexico City, Mexico
Hernández, M:
Univ Nacl Autonoma Mexico, Fac Ingn, Div Ingn Mecan & Ind DIMEI, Mexico City, Mexico
|