Phenotypic and Molecular Characterization of Candida albicans Isolates from Mexican Women with Vulvovaginitis
Por:
Díaz-Huerta, H, García-Salazar, E, Ramírez-Magaña, X, Martínez-Herrera, E, Pinto-Almazán, R, Betancourt-Cisneros, P, Duarte-Escalante, E, Reyes-Montes, MD, Hernández-Castro, R, Frías-De-León, MG
Publicada:
2 may 2025
Resumen:
Vulvovaginal candidiasis (VVC) is an opportunistic mycosis that affects women of reproductive age. The most frequent etiological agent is Candida albicans. The development of VVC depends on factors related to the host and the fungus. Among the factors related to Candida spp. are virulence factors, but genotype may also be involved. The objective of this study was to evaluate the ABC genotypes and extracellular hydrolytic enzyme production in C. albicans isolates obtained from Mexican women with vulvovaginitis to determine if there is a correlation between these characteristics that allows the fungus to invade and cause damage to the host. Forty-three yeast isolates were obtained from vaginal exudates from women with symptoms of infection. The isolates were identified by germ tube tests and by Cand PCR. The ABC genotype of the isolates identified as C. albicans was determined through the isolates' DNA amplification using the oligonucleotides CA-INT-R and CA-INT-L. The activity of esterase, phospholipase, proteinase, and hemolysin was evaluated in specific culture media. The correlation between extracellular enzyme production and genotype was analyzed using a two-way ANOVA and the Sidak comparison test. A total of 57.5% of the yeast isolates were identified as C. albicans. The genotypes identified were A (82.6%) and B (17.4%). The activity of esterase, phospholipase, proteinase, and hemolysin was very strong. No statistically significant difference was found between enzyme production and genotypes. In conclusion, genotype A predominates among C. albicans vaginal isolates. The production of extracellular hydrolytic enzymes was widely expressed in C. albicans vaginal isolates, but no correlation with genotype was found.
Filiaciones:
Díaz-Huerta, H:
Inst Politecn Nacl, Escuela Super Med, Programa Maestria Ciencias Salud, Plan San Luis & Diaz Miron S-N, Ciudad De Mexico 11340, Mexico
Hosp Reg Alta Especialidad Ixtapaluca, Inst Mexicano Seguro Social Bienestar IMSS BIENEST, Unidad Calidad & Riesgo Biol, Serv Salud, Carretera Fed Mexico Puebla Km 34-5, Ixtapaluca 56530, Estado De Mexic, Mexico
García-Salazar, E:
Hosp Reg Alta Especialidad Ixtapaluca, Unidad Invest Biomed, Lab Micol Mol, Inst Mexicano Seguro Social Bienestar IMSS BIENEST, Carretera Fed Mexico Puebla Km 34-5, Ixtapaluca 56530, Estado De Mexic, Mexico
Ramírez-Magaña, X:
Hosp Reg Alta Especialidad Ixtapaluca, Serv Ginecol & Obstet, Inst Mexicano Seguro Social Bienestar IMSS BIENEST, Carretera Fed Mexico Puebla Km 34-5, Ixtapaluca 56530, Estado De Mexic, Mexico
Martínez-Herrera, E:
Inst Politecn Nacl, Escuela Super Med, Secc Estudios Posgrad & Invest, Plan San Luis & Diaz Miron S-N, Ciudad De Mexico 11340, Mexico
Pinto-Almazán, R:
Inst Politecn Nacl, Escuela Super Med, Secc Estudios Posgrad & Invest, Plan San Luis & Diaz Miron S-N, Ciudad De Mexico 11340, Mexico
Betancourt-Cisneros, P:
Hosp Reg Alta Especialidad Ixtapaluca, Unidad Invest Biomed, Lab Micol Mol, Inst Mexicano Seguro Social Bienestar IMSS BIENEST, Carretera Fed Mexico Puebla Km 34-5, Ixtapaluca 56530, Estado De Mexic, Mexico
Duarte-Escalante, E:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ave Univ 3000,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Reyes-Montes, MD:
Univ Nacl Autonoma Mexico, Fac Med, Dept Microbiol & Parasitol, Ave Univ 3000,Ciudad Univ, Ciudad De Mexico 04510, Mexico
Hernández-Castro, R:
Hosp Manuel Gea Gonzalez, Dept Ecol Pathogen Agents, Calz Tlalpan 4800,Belisario Dominguez Secc 16, Ciudad De Mexico 14080, Mexico
Frías-De-León, MG:
Hosp Reg Alta Especialidad Ixtapaluca, Unidad Invest Biomed, Lab Micol Mol, Inst Mexicano Seguro Social Bienestar IMSS BIENEST, Carretera Fed Mexico Puebla Km 34-5, Ixtapaluca 56530, Estado De Mexic, Mexico
|