CD38 Expression in a Subset of Memory T Cells Is Independent of Cell Cycling as a Correlate of HIV Disease Progression
Por:
Würsch D., Ormsby C.E., Romero-Rodríguez D.P., Olvera-García G., Zúñiga J., Jiang W., Pérez-Patrigeon S., Espinosa E.
Publicada:
1 ene 2016
Resumen:
In order to determine if the expression of the activation marker CD38 can correlate with HIV disease progression independently of cycling, we performed a cluster-based multivariate correlation analysis of total circulating CD4+ T cell counts and viral loads with frequencies of CD38 and Ki67 expression on CD4+ lymphocytes from patients with untreated HIV infection, stratified in maturation subpopulations, and subpopulation subsets defined by the expression of CXCR5, CXCR3, and CCR4. The frequencies of the activated phenotypes %CD38+ Ki67- and %CD38+ Ki67+ of the CXCR5- CXCR3- CCR4+ ("pre-Th2") central memory (T C M) cell subset clustered together, comprising a significant negative correlate of total circulating CD4+ T cell counts and a positive correlate of viral load in multivariate analysis. Frequency of cycling-uncoupled CD38 expression in "pre-Th2" T C M cells was a negative correlate of total circulating CD4+ T cell counts in univariate analysis, which was not the case of their %CD38+ Ki67+. CXCR5+ CXCR3- CCR4- T C M cells were underrepresented in patients, and their absolute counts correlated negatively with their %CD38+ Ki67- but not with their % CD38+ Ki67+. Our results may imply that CD38 expression either reflects or participates in pathogenic mechanisms of HIV disease independently of cell cycling. © 2016 Daniela Würsch et al.
Filiaciones:
Würsch D.:
Department for Research in Immunology, National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, Mexico City, DF, 14080, Mexico
Ormsby C.E.:
Center for Research on Infectious Diseases (CIENI), National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, Mexico City, DF, 14080, Mexico
Romero-Rodríguez D.P.:
Flow Cytometry Core Facility, National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, Mexico City, DF, 14080, Mexico
Olvera-García G.:
Department for Research in Immunology, National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, Mexico City, DF, 14080, Mexico
Zúñiga J.:
Department for Research in Immunology, National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, Mexico City, DF, 14080, Mexico
Jiang W.:
Department of Medicine, Department of Immunology and Microbiology, Medical University of South Carolina, 173 Ashley Avenue, Basic Science Building, Room 208D, Charleston, 29425, SC, United States
Pérez-Patrigeon S.:
Department of Infectious Diseases, National Institute for Medical Sciences and Nutrition, Vasco de Quiroga 15, Mexico City, DF, 14080, Mexico
Espinosa E.:
Department for Research in Immunology, National Institute for Respiratory Diseases, Calzada de Tlalpan 4502, Mexico City, DF, 14080, Mexico
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