Massive Sequencing of V3-V4 Hypervariable Region in Pyogenic Liver Abscesses Reveals the Presence of Unusual Bacteria Not Detected by Classical Culture Methods
Por:
Fernández-Sánchez V., Plascencia-Nieto E.S., Cureño-Díaz M.A., Durán-Manuel E.M., Rodríguez-Tovar A.V., Calzada-Mendoza C.C., Cruz-Cruz C., Loyola-Cruz M.Á., Álvarez-Sánchez M.E., Bravata-Alcántara J.C., Vásquez-Jiménez E., Gutiérrez-Muñoz V.H., Blanco-Hernández D.M.R., Nicolás-Sayago L., Rojas-Bernabé A., García-Hernández O., Gómez-Zamora E., Ruíz-Valdés M., Castro-Escarpulli G., Bello-López J.M.
Publicada:
1 ene 2025
Resumen:
Pyogenic liver abscesses (PLAs) are serious infections in which doctors often fail in identifying the causative agent due to microbiological limitations. These limitations in detecting uncommon pathogens complicate the treatment and recovery. Molecular techniques, like massive sequencing, enable the detection of uncommon pathogens and highlight the shortcomings of traditional cultures. The aim of this work was to characterise the bacterial composition of PLAs through massive sequencing of the V3-V4 hypervariable region of the 16S rRNA gene in cases where conventional culture methods were negative. Purulent material was collected from three patients with PLAs at Hospital Juárez de México. Classical and molecular microbiological cultures were performed in parallel. Metagenomic DNA was extracted and massively sequenced (16S rRNA gene) using the Illumina MiSeq platform. A bioinformatic analysis was performed to determine the diversity at six different taxa levels and the relative abundances. The culture methods were not sufficient to detect the causative agent of the PLAs. However, the massive sequencing revealed the causative agents of the monomicrobial and polymicrobial infectious foci, with Gardnerella vaginalis, Lactobacillus iners, and Prevotella timonensis as the dominant bacteria. The massive sequencing revealed the presence of unusual pathogens that traditional culture failed to detect. There is an immediate need for molecular or comprehensive microbiological culture techniques to search for unusual bacteria in the diagnosis of PLAs. © 2025 by the authors.
Filiaciones:
Hospital Juárez de México, Mexico City, 07760, Mexico
Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Mexico City, 54090, Mexico
Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina, Instituto Politécnico Nacional, Mexico City, 11340, Mexico
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, 11340, Mexico
Laboratorio de Patogénesis Celular y Molecular Humana y Veterinaria, Posgrado en Ciencias Genómicas, Universidad Autónoma de la Ciudad de México, Mexico City, 03100, Mexico
División de Investigación, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, 54090, Mexico
gold, All Open Access; Gold Open Access
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