Towards Characterization of Hass Avocado Peel and Pulp Proteome during Postharvest Shelf Life
Por:
Camacho-Vázquez, C, Elizalde-Contreras, JM, Reyes-Soria, FA, Monribot-Villanueva, JL, Guerrero-Analco, JA, Juarez-Escobar, J, Velázquez-López, O, Meza-Menchaca, T, Bojórquez-Velázquez, E, Zamora-Briseño, JA, Ramirez-Vazquez, M, Barrenechea, GAG, Ibarra-Laclette, E, Ruiz-May, E
Publicada:
1 dic 2024
Resumen:
In recent years, avocados have gained worldwide popularity as a nutritive food. This trend is causing a rise in the production of this fruit, which is accompanied by several problems associated with monocultural practices. Despite massive economic gains, limited molecular and structural information has been generated about avocado ripening. In fact, limited studies have attempted to unravel the proteome complexity dynamics of avocado fruit. We therefore conducted a comparative proteomics study on avocado peel and pulp during the postharvest shelf life using tandem mass tag synchronous precursor selection triple-stage mass spectrometry. We identified 3161 and 1128 proteins in the peel and pulp, respectively. Peels exhibited major over-accumulation of proteins associated with water deprivation and oxidative stress, along with abscisic acid biosynthesis. Ethylene, jasmonic acid, phenylpropanoid, and flavonoid biosynthesis pathways were activated. Structurally, we observed the accumulation of lignin and a reduction in cuticular thickness, which coincides with the reduction in the levels of long-chain acyl-coenzyme A synthetase and a marginal increase in 10,16-dihydroxyhexadecanoic acid. Our study sheds light on the association of proteome modulation with the structural features of Hass avocado. Its detailed characterization will provide an alternative for better preservation during the postharvest period.
Filiaciones:
Camacho-Vázquez, C:
Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, El Haya 91073, Xalapa, Mexico
Elizalde-Contreras, JM:
Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, El Haya 91073, Xalapa, Mexico
Reyes-Soria, FA:
Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, El Haya 91073, Xalapa, Mexico
Monribot-Villanueva, JL:
Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, El Haya 91073, Xalapa, Mexico
Guerrero-Analco, JA:
Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, El Haya 91073, Xalapa, Mexico
Juarez-Escobar, J:
Univ Veracruzana, Fac Biol, Zona Univ, Xalapa 91090, Mexico
Velázquez-López, O:
Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, El Haya 91073, Xalapa, Mexico
Meza-Menchaca, T:
Univ Veracruzana, Fac Med, Lab Genom Humana, Med & Odontol S-N,Col Unidad Bosque, Xalapa 91010, Mexico
Bojórquez-Velázquez, E:
Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, El Haya 91073, Xalapa, Mexico
Zamora-Briseño, JA:
Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, El Haya 91073, Xalapa, Mexico
Ramirez-Vazquez, M:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Microscopia, Edificio A PB,Circuito Interior,Ave Univ 3000,Ciud, Ciudad Mexico 04510, Mexico
Barrenechea, GAG:
Ctr Invest Cient Yucatan, Carretera Sierra Papacal Chuburna Puerto,Parque Ci, Merida 97302, Mexico
Ibarra-Laclette, E:
Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, El Haya 91073, Xalapa, Mexico
Ruiz-May, E:
Inst Ecol AC, Red Estudios Mol Avanzados, Carretera Antigua Coatepec 351, El Haya 91073, Xalapa, Mexico
gold, All Open Access; Gold Open Access
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