Food grade titanium dioxide induced endoplasmic reticulum stress in colon cells: Comparison between normal and colorectal carcinoma cells
Por:
Uribe-García, A, Medina-Reyes, EI, Flores-Reyes, CA, Zagal-Salinas, AA, Ispanixtlahuatl-Meraz, O, Delgado-Armenta, E, Santibáñez-Andrade, M, Flores, CM, Sánchez-Pérez, Y, García-Cuéllarb, CM, Chirino, YI
Publicada:
1 ene 2025
Ahead of Print:
1 nov 2024
Resumen:
Background: Food-grade titanium dioxide (E171) has been under scrutiny in the last decade since its possible adverse effects; however, the cellular mechanisms underlying E171 toxicity have not been thoroughly described. Aim: We aimed to compare the effects of E171 on endoplasmic reticulum (ER) homeostasis in normal and cancer colon cells. Experimental design: We exposed normal, carcinoma, and adenocarcinoma cells to 0.1, 1, 10, 50 and 100 mu g/cm2 of E171 for 24, 48 and 72 h, and we evaluated ER stress, cell viability, titanium uptake, intracellular calcium concentration, and gene expression related to unfolded protein response (UPR) and chaperone pathways. Results: Cell viability decreased only after 72 h of exposure to 100 mu g/cm2 of E171. Adenocarcinoma cells internalized higher titanium amounts than normal and carcinoma cells, but the effects in ER distribution, intracellular calcium concentration, and gene expression were similar among the three cell lines. The expression of UPR and chaperone pathways were downregulated at the lowest concentrations but upregulated at the highest concentrations in the three cell lines. Conclusion: E171 induces ER stress through alterations in ER distribution, intracellular calcium, and UPR and chaperone protein pathways.
Filiaciones:
Uribe-García, A:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Lab Carcinogenesis & Toxicol, Ave Barrios 1, Tlalnepantla de Baz 54090, Estado de Mexic, Mexico
Medina-Reyes, EI:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Lab Carcinogenesis & Toxicol, Ave Barrios 1, Tlalnepantla de Baz 54090, Estado de Mexic, Mexico
Flores-Reyes, CA:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Lab Carcinogenesis & Toxicol, Ave Barrios 1, Tlalnepantla de Baz 54090, Estado de Mexic, Mexico
Zagal-Salinas, AA:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Lab Carcinogenesis & Toxicol, Ave Barrios 1, Tlalnepantla de Baz 54090, Estado de Mexic, Mexico
Ispanixtlahuatl-Meraz, O:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Lab Carcinogenesis & Toxicol, Ave Barrios 1, Tlalnepantla de Baz 54090, Estado de Mexic, Mexico
Delgado-Armenta, E:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Lab Carcinogenesis & Toxicol, Ave Barrios 1, Tlalnepantla de Baz 54090, Estado de Mexic, Mexico
Santibáñez-Andrade, M:
Inst Nacl Cancerol, Subdirecc Invest Bas, San Fernando 22, Mexico City 14080, Mexico
Flores, CM:
Iztacala UNAM, Lab Fisiol Vegetal, UBIPRO Lab Nacl Salud, Fac Estudios Super, Tlalnepantla de Baz, Estado de Mexic, Mexico
Sánchez-Pérez, Y:
Inst Nacl Cancerol, Subdirecc Invest Bas, San Fernando 22, Mexico City 14080, Mexico
García-Cuéllarb, CM:
Inst Nacl Cancerol, Subdirecc Invest Bas, San Fernando 22, Mexico City 14080, Mexico
Chirino, YI:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Unidad Biomed, Lab Carcinogenesis & Toxicol, Ave Barrios 1, Tlalnepantla de Baz 54090, Estado de Mexic, Mexico
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