Microtomographic and histological evaluation of two bioceramics as pulp capping agents in vivo;
Por:
Torres-Flamenco D., Jiménez-Castellanos R., Pérez-Sánchez L., Llaguno-Munive M., González-Alva P., Vázquez-Vázquez F.C., Ortiz-Magdaleno M., Serrano-Bello J.
Publicada:
1 ene 2024
Categoría:
Medicine (miscellaneous)
Resumen:
Maintaining pulp vitality and function is a priority of the medicaments employed in pulp therapy to preserve tooth integrity. Aim: This study evaluated inflammatory response and reparative dentin bridge formation after direct pulp capping with two different bioceramics. Materials and Method: This was an in vivo controlled experimental study on 12 male Wistar rats. Pulpotomies were performed and the exposed pulps were capped with Biodentine or Neo MTA. After 15, 45 and 90 days, maxillary segments were obtained and prepared for histologic analysis and Micro-CT. Hounsfield Units (HU) were quantified. Results: Micro-CT analysis showed greater mineralization at 90 days with Neo MTA than with Biodentine. HU did not differ significantly (p >0.05) between molars treated with Biodentine and Neo MTA at 15 and 45 days, but at 90 days, there was statistically significant difference (p <0.05) between them. Reparative dentin was observed near the pulp exposure and canal orifice with both bioceramics. At 45 and 90 days, molars treated with Neo MTA showed mineralized tissue filling the canal orifice. Molars treated with Biodentine showed mineralized tissue and dentin bridge at the site of exposure at 45 days, and total pulp exposure coverage and mineralized dentin matrix at 90 days. Conclusions: Biodentine and Neo MTA induce the formation of reparative dentin bridge after 45 days with inflammatory cell infiltrate. © 2024, Acta Odontologica Latinoamericana. All rights reserved.
Filiaciones:
Torres-Flamenco D.:
Departamento de Endodoncia, División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico
Jiménez-Castellanos R.:
Departamento de Endodoncia, División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico
Pérez-Sánchez L.:
Laboratorio de Bioingeniería de Tejidos, División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico
Llaguno-Munive M.:
Laboratorio de Física Médica, Subdirección de Investigación Básica, Instituto Nacional de Cancerología, Mexico
González-Alva P.:
Laboratorio de Bioingeniería de Tejidos, División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico
Vázquez-Vázquez F.C.:
Laboratorio de Investigación de Materiales Dentales y Biomateriales, División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico
Ortiz-Magdaleno M.:
Facultad de Estomatología, Universidad Autónoma de San Luis Potosí, Mexico
Serrano-Bello J.:
Laboratorio de Bioingeniería de Tejidos, División de Estudios de Posgrado e Investigación, Facultad de Odontología, Universidad Nacional Autónoma de México, Mexico
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