Anandamide and WIN 55212–2 Afford Protection in Rat Brain Mitochondria in a Toxic Model Induced by 3-Nitropropionic Acid: an In Vitro Study
Por:
Maya-López M., Monsalvo-Maraver L.A., Delgado-Arzate A.L., Olivera-Pérez C.I., El-Hafidi M., Silva-Palacios A., Medina-Campos O., Pedraza-Chaverri J., Aschner M., Tinkov A.A., Túnez I., Retana-Márquez S., Zazueta C., Santamaría A.
Publicada:
1 ene 2024
Ahead of Print:
1 feb 2024
Resumen:
Mitochondrial dysfunction plays a key role in the development of neurodegenerative disorders. In contrast, the regulation of the endocannabinoid system has been shown to promote neuroprotection in different neurotoxic paradigms. The existence of an active form of the cannabinoid receptor 1 (CB1R) in mitochondrial membranes (mitCB1R), which might exert its effects through the same signaling mechanisms as the cell membrane CB1R, has been shown to regulate mitochondrial activity. Although there is evidence suggesting that some cannabinoids may induce protective effects on isolated mitochondria, substantial evidence on the role of cannabinoids in mitochondria remains to be explored. In this work, we developed a toxic model of mitochondrial dysfunction induced by exposure of brain mitochondria to the succinate dehydrogenase inhibitor 3-nitropropionic acid (3-NP). Mitochondria were also pre-incubated with the endogenous agonist anandamide (AEA) and the synthetic CB1R agonist WIN 55212–2 to evaluate their protective effects. Mitochondrial reduction capacity, reactive oxygen species (ROS) formation, and mitochondrial swelling were assessed as toxic markers. While 3-NP decreased the mitochondrial reduction capacity and augmented mitochondrial ROS formation and swelling, both AEA and WIN 55212–2 ameliorated these toxic effects. To explore the possible involvement of mitCB1R activation on the protective effects of AEA and WIN 55212–2, mitochondria were also pre-incubated in the presence of the selective CB1R antagonist AM281, which completely reverted the protective effects of the cannabinoids to levels similar to those evoked by 3-NP. These results show partial protective effects of cannabinoids, suggesting that mitCB1R activation may be involved in the recovery of compromised mitochondrial activity, related to reduction of ROS formation and further prevention of mitochondrial swelling. © The Author(s) 2024. corrected publication 2024.
Filiaciones:
Doctorado en Ciencias Biológicas y de La Salud, Universidad Autónoma Metropolitana, Mexico City, 09310, Mexico
Facultad de Ciencias, Universidad Nacional Autónoma de México, México City, 04510, Mexico
Departamento de Biomedicina Cardiovascular, Instituto Nacional de Cardiología, SSA, Mexico City, 14080, Mexico
Laboratorio F-315, Departamento de Biología, Facultad de Química, Universidad Autónoma de México, Mexico City, 04510, Mexico
Department of Molecular Pharmacology, Albert Einstein College of Medicine, Bronx, 10461, NY, United States
Laboratory of Molecular Dietetics, IM Sechenov First Moscow State Medical University (Sechenov University), Moscow, 119435, Russian Federation
Department of Human Ecology and Bioelementology, and Department of Medical Elementology, Peoples’ Friendship University of Russia (RUDN University), Moscow, 117198, Russian Federation
Instituto de Investigaciones Biomédicas Maimonides de Córdoba (IMIBIC), Córdoba, Spain
Departamento de Bioquímica y Biología Molecular, Facultad de Medicina y Enfermería, Universidad de Córdoba, Córdoba, Spain
Red Española de Excelencia en Estimulación Cerebral (REDESTIM), Córdoba, 14071, Spain
Departamento de Biología de La Reproducción, Universidad Autónoma Metropolitana-Iztapalapa, Mexico City, 09310, Mexico
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