The activation of D2-like dopamine receptors increases NMDA currents in the dorsal raphe serotonergic neurons
Por:
Galindo-Charles L., Reyes-Legorreta C., Garduño J., Galarraga E., Tapia D., Hernández-López S.
Publicada:
1 ene 2024
Categoría:
Neuroscience (miscellaneous)
Resumen:
The dorsal raphe nucleus (DRN) receives dopaminergic inputs from the ventral tegmental area (VTA). Also, the DRN contains a small population of cells that express dopamine (DRNDA neurons). However, the physiological role of dopamine (DA) in the DRN and its interaction with serotonergic (5-HT) neurons is poorly understood. Several works have reported moderate levels of D1, D2, and D3 DA receptors in the DRN. Furthermore, it was found that the activation of D2 receptors increased the firing of putative 5-HT neurons. Other studies have reported that D1 and D2 dopamine receptors can interact with glutamate NMDA receptors, modulating the excitability of different cell types. In the present work, we used immunocytochemical techniques to determine the kind of DA receptors in the DRN. Additionally, we performed electrophysiological experiments in brainstem slices to study the effect of DA agonists on NMDA-elicited currents recorded from identified 5-HT DRN neurons. We found that D2 and D3 but not D1 receptors are present in this nucleus. Also, we demonstrated that the activation of D2-like receptors increases NMDA-elicited currents in 5-HT neurons through a mechanism involving phospholipase C (PLC) and protein kinase C (PKC) enzymes. Possible physiological implications related to the sleep-wake cycle are discussed. © 2024 The Authors
Filiaciones:
Galindo-Charles L.:
Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, 04510, Mexico
Reyes-Legorreta C.:
Laboratorio de Neuroprotección, Instituto Nacional de Rehabilitación-LGII, Ciudad de México, 14389, Mexico
Garduño J.:
Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, 04510, Mexico
Galarraga E.:
División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, 04510, Mexico
Tapia D.:
División de Neurociencias, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, 04510, Mexico
Hernández-López S.:
Departamento de Fisiología, Facultad de Medicina, Universidad Nacional Autónoma de México (UNAM), Ciudad de México, 04510, Mexico
hybrid, All Open Access; Hybrid Gold Open Access
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