Proteomic Analysis of Heloderma horridum horridum Venom: Assessment to Its Transcriptome and Newfound Proteins
Por:
Lino-López, GJ, Ruiz-May, E, Elizalde-Contreras, JM, Jiménez-Vargas, JM, Rodríguez-Vázquez, A, González-Carrillo, G, Bojórquez-Velázquez, E, García-Villalvazo, PE, Bermúdez-Guzmán, MD, Zatarain-Palacios, R, Vázquez-Vuelvas, OF, Valdez-Velázquez, LL, Corzo, G
Publicada:
22 jul 2024
Ahead of Print:
1 jul 2024
Resumen:
Heloderma horridum horridum, a venomous reptile native to America, has a venom with potential applications in treating type II diabetes. In this work, H. h. horridum venom was extracted, lyophilized, and characterized using enzymatic assays for hyaluronidase, phospholipase, and protease. Proteomic analysis of the venom was conducted employing bottom-up/shotgun approaches, SDS-PAGE, high-pH reversed-phase chromatography, and fractionation of tryptic peptides using nano-LC-MS/MS. The proteins found in H. h. horridum venom were reviewed according to the classification of the transcriptome previously reported. The proteomic approach identified 101 enzymes, 36 other proteins, 15 protein inhibitors, 11 host defense proteins, and 1 toxin, including novel venom components such as calcium-binding proteins, phospholipase A2 inhibitors, serpins, cathepsin, subtilases, carboxypeptidase-like, aminopeptidases, glycoside hydrolases, thioredoxin transferases, acid ceramidase-like, enolase, multicopper oxidases, phosphoglucose isomerase (PGI), fructose-1,6-bisphosphatase class 1, pentraxin-related, peptidylglycine alpha-hydroxylating monooxygenase/peptidyl-hydroxyglycine alpha-amidating lyase, carbonic anhydrase, acetylcholinesterase, dipeptidylpeptidase, and lysozymes. These findings contribute to understanding the venomous nature of H. h. horridum and highlight its potential as a source of bioactive compounds. Data are available via PRoteomeXchange with the identifier PXD052417.
Filiaciones:
Lino-López, GJ:
Univ Colima, Fac Ciencias Quim, Colima 28400, Mexico
CNRF DGSV SENAS SADER, Dept Control Biol, Colima 28110, Mexico
Ruiz-May, E:
Inst Ecol, Xalapa 91073, Veracruz, Mexico
Elizalde-Contreras, JM:
Inst Ecol, Xalapa 91073, Veracruz, Mexico
Jiménez-Vargas, JM:
Univ Colima, Fac Ciencias Quim, Colima 28400, Mexico
Rodríguez-Vázquez, A:
Ctr Conservac Vida Silvestre El Palapo, Colima 28400, Mexico
González-Carrillo, G:
Tecnol Nacl Mexico ITJMMPyH, UA Tamazula, Tamazula De Gordiano 49650, Jalisco, Mexico
Bojórquez-Velázquez, E:
Inst Ecol, Xalapa 91073, Veracruz, Mexico
García-Villalvazo, PE:
Univ Colima, Fac Ciencias Quim, Colima 28400, Mexico
Bermúdez-Guzmán, MD:
INIFAP, Colima 28100, Mexico
Zatarain-Palacios, R:
Univ Colima, Fac Ciencias Quim, Colima 28400, Mexico
Vázquez-Vuelvas, OF:
Univ Colima, Fac Ciencias Quim, Colima 28400, Mexico
Valdez-Velázquez, LL:
Univ Colima, Fac Ciencias Quim, Colima 28400, Mexico
Corzo, G:
Univ Nacl Autonoma Mexico, Inst Biotecnol, Cuernavaca 62210, Morelos, Mexico
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