Acute Effects of Dietary Protein Consumption on the Postprandial Metabolic Response, Amino Acid Levels and Circulating MicroRNAs in Patients with Obesity and Insulin Resistance
Por:
Hernández-Gómez K.G., Velázquez-Villegas L.A., Granados-Portillo O., Avila-Nava A., González-Salazar L.E., Serralde-Zúñiga A.E., Palacios-González B., Pichardo-Ontiveros E., Guizar-Heredia R., López-Barradas A.M., Sánchez-Tapia M., Larios-Serrato V., Olin-Sandoval V., Díaz-Villaseñor A., Medina-Vera I., Noriega L.G., Alemán-Escondrillas G., Ortiz-Ortega V.M., Torres N., Tovar A.R., Guevara-Cruz M.
Publicada:
1 ene 2024
Resumen:
The post-nutritional intervention modulation of miRNA expression has been previously investigated; however, post-acute dietary-ingestion-related miRNA expression dynamics in individuals with obesity and insulin resistance (IR) are unknown. We aimed to determine the acute effects of protein ingestion from different dietary sources on the postprandial metabolic response, amino acid levels, and circulating miRNA expression in adults with obesity and IR. This clinical trial included adults with obesity and IR who consumed (1) animal-source protein (AP; calcium caseinate) or (2) vegetable-source protein (VP; soy protein isolate). Glycaemic, insulinaemic, and glucagon responses, amino acid levels, and exosomal microRNAs isolated from plasma were analysed. Post-AP ingestion, the area under the curve (AUC) of insulin (p = 0.04) and the plasma concentrations of branched-chain (p = 0.007) and gluconeogenic (p = 0.01) amino acids increased. The effects of different types of proteins on the concentration of miRNAs were evaluated by measuring their plasma circulating levels. Compared with the baseline, the AP group presented increased circulating levels of miR-27a-3p, miR-29b-3p, and miR-122-5p (p < 0.05). Subsequent analysis over time at 0, 30, and 60 min revealed the same pattern and differences between treatments. We demonstrated that a single dose of dietary protein has acute effects on hormonal and metabolic regulation and increases exosomal miRNA expression in individuals with obesity and IR. © 2024 by the authors.
Filiaciones:
Departamento de Fisiología de la Nutrición, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, 14080, Mexico
Department of Obstetrics, Gynecology and Reproductive Sciences, University of Manitoba, Winnipeg, R3T 2N2, MB, Canada
Servicio de Nutriología Clínica, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City, 14080, Mexico
Laboratorio de Envejecimiento Saludable del INMEGEN en el Centro de Investigación Sobre el Envejecimiento, Mexico City, 14330, Mexico
Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Mexico City, 11340, Mexico
Departamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Instituto Politécnico Nacional, Mexico City, 07360, Mexico
Departamento de Medicina Genómica y Toxicología Ambiental, Instituto de Investigaciones Biomédicas, UNAM, Mexico City, 04510, Mexico
Departamento de Metodología de la Investigación, Instituto Nacional de Pediatría, Mexico City, 04530, Mexico
gold, All Open Access; Gold Open Access
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