Silybin restores glucose uptake after tumour necrosis factor-alpha and lipopolysaccharide stimulation in 3T3-L1 adipocytes
Por:
Butanda-Nuñez, A, Rodríguez-Cortés, O, Ramos-Martínez, E, Cerbón, MA, Escobedo, G, Chavarría, A
Publicada:
31 dic 2024
Resumen:
Obesity is associated with a low-grade chronic inflammatory process characterized by higher circulating TNF alpha levels, thus contributing to insulin resistance. This study evaluated the effect of silybin, the main bioactive component of silymarin, which has anti-inflammatory properties, on TNF alpha levels and its impact on glucose uptake in the adipocyte cell line 3T3-L1 challenged with two different inflammatory stimuli, TNF alpha or lipopolysaccharide (LPS). Silybin's pre-treatment effect was evaluated in adipocytes pre-incubated with silybin (30 or 80 mu M) before challenging with the inflammatory stimuli (TNF alpha or LPS). For the post-treatment effect, the adipocytes were first challenged with the inflammatory stimuli and then post-treated with silybin. After treatments, TNF alpha production, glucose uptake, and GLUT4 protein expression were determined. Both inflammatory stimuli increased TNF alpha secretion, diminished GLUT4 expression, and significantly decreased glucose uptake. Silybin 30 mu M only reduced TNF alpha secretion after the LPS challenge. Silybin 80 mu M as post-treatment or pre-treatment decreased TNF alpha levels, improving glucose uptake. However, glucose uptake enhancement induced by silybin did not depend on GLUT4 protein expression. These results show that silybin importantly reduced TNF alpha levels and upregulates glucose uptake, independently of GLUT4 protein expression.
Filiaciones:
Butanda-Nuñez, A:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Med Expt, Mexico City, Mexico
Rodríguez-Cortés, O:
Inst Politecn Nacl, Escuela Super Med, Lab 103, SEPI, Mexico City, Mexico
Ramos-Martínez, E:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Med Expt, Mexico City, Mexico
Cerbón, MA:
Univ Nacl Autonoma Mexico, Fac Quim, Unidad Invest Reprod Humana, Inst Nacl Perinatol, Mexico City, Mexico
Escobedo, G:
Hosp Gen Mex Dr Eduardo Liceaga, Lab Prote & Metabol, Mexico City, Mexico
Chavarría, A:
Univ Nacl Autonoma Mexico, Fac Med, Unidad Med Expt, Mexico City, Mexico
gold, All Open Access; Gold Open Access
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