Biochemical characterization and gene structure analysis of the 24-kDa glutathione transferase sigma from Taenia solium
Por:
Miranda-Blancas R., Rodríguez-Lima O., García-Gutiérrez P., Flores-López R., Jiménez L., Zubillaga R.A., Rudiño-Piñera E., Landa A.
Publicada:
1 ene 2024
Categoría:
Biochemistry, genetics and molecular biology (miscellaneous)
Resumen:
Taenia solium can cause human taeniasis and/or cysticercosis. The latter can in some instances cause human neurocysticercosis which is considered a priority in disease-control strategies and the prevention of mental health problems. Glutathione transferases are crucial for the establishment and long-term survival of T. solium; therefore, we structurally analyzed the 24-kDa glutathione transferase gene (Ts24gst) of T. solium and biochemically characterized its product. The gene promoter showed potential binding sites for transcription factors and xenobiotic regulatory elements. The gene consists of a transcription start site, four exons split by three introns, and a polyadenylation site. The gene architecture is conserved in cestodes. Recombinant Ts24GST (rTs24GST) was active and dimeric. Anti-rTs24GST serum showed slight cross-reactivity with human sigma-class GST. A 3D model of Ts24GST enabled identification of putative residues involved in interactions of the G-site with GSH and of the H-site with CDNB and prostaglandin D2. Furthermore, rTs24GST showed optimal activity at 45 °C and pH 9, as well as high structural stability in a wide range of temperatures and pHs. These results contribute to the better understanding of this parasite and the efforts directed to fight taeniasis/cysticercosis. © 2024 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
Filiaciones:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico
Departamento de Química, Universidad Autónoma Metropolitana-Iztapalapa, Mexico, Mexico
Posgrado en Ciencias Biológicas Unidad de Posgrado, Universidad Nacional Autónoma de México, Mexico
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
All Open Access; Gold Open Access
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