High-Throughput Screening Method Using Escherichia coli Keio Mutants for Assessing Primary Damage Mechanism of Antimicrobials
Por:
Martínez-Álvarez J.A., Vicente-Gómez M., García-Contreras R., Wood T.K., Ramírez Montiel F.B., Vargas-Maya N.I., España-Sánchez B.L., Rangel-Serrano Á., Padilla-Vaca F., Franco B.
Publicada:
1 ene 2024
Resumen:
The Escherichia coli Keio mutant collection has been a tool for assessing the role of specific genes and determining their role in E. coli physiology and uncovering novel functions. In this work, specific mutants in the DNA repair pathways and oxidative stress response were evaluated to identify the primary targets of silver nanoparticles (NPs) and their mechanism of action. The results presented in this work suggest that NPs mainly target DNA via double-strand breaks and base modifications since the recA, uvrC, mutL, and nfo mutants rendered the most susceptible phenotype, rather than involving the oxidative stress response. Concomitantly, during the establishment of the control conditions for each mutant, the katG and sodA mutants showed a hypersensitive phenotype to mitomycin C, an alkylating agent. Thus, we propose that KatG catalase plays a key role as a cellular chaperone, as reported previously for the filamentous fungus Neurospora crassa, a large subunit catalase. The Keio collection mutants may also be a key tool for assessing the resistance mechanism to metallic NPs by using their potential to identify novel pathways involved in the resistance to NPs. © 2024 by the authors.
Filiaciones:
Martínez-Álvarez J.A.:
Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, 36050, Mexico
Vicente-Gómez M.:
Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, 36050, Mexico
García-Contreras R.:
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, 04510, Mexico
Wood T.K.:
Department of Chemical Engineering, Pennsylvania State University, University Park, 16802-4400, PA, United States
Ramírez Montiel F.B.:
Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, 36050, Mexico
Vargas-Maya N.I.:
Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, 36050, Mexico
España-Sánchez B.L.:
Centro de Investigación y Desarrollo Tecnológico en Electroquímica CIDETEQ S.C., Parque Tecnológico Querétaro s/n, Sanfandila, Pedro Escobedo, Querétaro, 76703, Mexico
Rangel-Serrano Á.:
Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, 36050, Mexico
Padilla-Vaca F.:
Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, 36050, Mexico
Franco B.:
Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, 36050, Mexico
gold, All Open Access; Gold Open Access
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