Aluminum enhances the oxidative damage of ZnO NMs in the human neuroblastoma SH-SY5Y cell line
Por:
Jimenez-Chavez A., Pedroza-Herrera G., Betancourt-Reyes I., De Vizcaya Ruiz A., Masuoka-Ito D., Zapien J.A., Medina-Ramirez I.E.
Publicada:
1 ene 2024
Resumen:
Bare and doped zinc oxide nanomaterials (ZnO NMs) are of great interest as multifunctional platforms for biomedical applications. In this study, we systematically investigate the physicochemical properties of Aluminum doped ZnO (AZO) and its bio-interactions with neuroblastoma (SH-SY5Y) and red blood (RBCs) cells. We provide a comprehensive chemical and structural characterization of the NMs. We also evaluated the biocompatibility of AZO NMs using traditional toxicity assays and advanced microscopy techniques. The toxicity of AZO NMs towards SH-SY5Y cells, decreases as a function of Al doping but is higher than the toxicity of ZnO NMs. Our results show that N-acetyl cysteine protects SH-SY5Y cells against reactive oxygen species toxicity induced by AZO NMs. ZnO and AZO NMs do not exert hemolysis in human RBCs at the doses that cause toxicity (IC50) in neuroblastoma cells. The Atomic force microscopy qualitative analysis of the interaction of SH-SY5Y cells with AZO NMs shows evidence that the affinity of the materials with the cells results in morphology changes and diminished interactions between neighboring cells. The holotomographic microscopy analysis demonstrates NMs' internalization in SH-SY5Y cells, changes in their chemical composition, and the role of lipid droplets in the clearance of toxicants. Graphical Abstract: (Figure presented.). © The Author(s) 2024.
Filiaciones:
Jimenez-Chavez A.:
Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados de IPN (CINVESTAV-IPN), Ciudad de Mexico, Mexico
Pedroza-Herrera G.:
Department of Chemistry, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ags, Aguascalientes, Mexico
Betancourt-Reyes I.:
Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México, México, Mexico
De Vizcaya Ruiz A.:
Departamento de Toxicología, Centro de Investigación y de Estudios Avanzados de IPN (CINVESTAV-IPN), Ciudad de Mexico, Mexico
Department of Environmental and Occupational Health, Program in Public Health, Susan and Henry Samueli College of Health Sciences, University of California Irvine, Irvine, CA, United States
Masuoka-Ito D.:
Department of Stomatology, Universidad Autónoma de Aguascalientes. Av. Universidad 940, Ags, Aguascalientes, Mexico
Zapien J.A.:
Department of Materials Science and Engineering, City University of Hong Kong, Hong Kong
Medina-Ramirez I.E.:
Department of Chemistry, Universidad Autónoma de Aguascalientes, Av. Universidad 940, Ags, Aguascalientes, Mexico
gold, All Open Access; Gold Open Access
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