Zika Virus-Infected Monocyte Exosomes Mediate Cell-to-Cell Viral Transmission
Por:
Martínez-Rojas, PP, Monroy-Martínez, V, Agredano-Moreno, LT, Jiménez-García, LF, Ruiz-Ordaz, BH
Publicada:
1 ene 2024
Resumen:
Zika fever is a reemerging arthropod-borne viral disease; however, Zika virus (ZIKV) can be transmitted by other, non-vector means. Severe Zika fever is characterized by neurological disorders, autoimmunity, or congenital Zika syndrome. Monocytes are primary ZIKV targets in humans and, in response to infection, release extracellular vesicles like exosomes. Exosomes mediate intercellular communication and are involved in the virus's ability to circumvent the immune response, promoting pathological processes. This study aimed to evaluate the role of monocyte exosomes in cell-to-cell viral transmission. We isolated exosomes from ZIKV-infected monocytes (Mo exo ZIKV) by differential ultracentrifugation and identified them by nanoparticle tracking analysis; transmission electron microscopy; and CD63, CD81, TSG101, and Alix detection by cytofluorometry. Purified exosome isolates were obtained by uncoupling from paramagnetic beads or by treatment with UV radiation and RNase A. We found that Mo exo ZIKV carry viral RNA and E/NS1 proteins and that their interaction with naive cells favors viral transmission, infection, and cell differentiation/activation. These data suggest that Mo exo ZIKV are an efficient alternative pathway for ZIKV infection. Knowledge of these mechanisms contributes to understanding the pathogenesis of severe disease and to the development of new vaccines and therapies.
Filiaciones:
Martínez-Rojas, PP:
Univ Nacl Autonoma Mexico, Dept Biol Mol & Biotecnol, Inst Invest Biomed, Mexico City 04510, Mexico
Monroy-Martínez, V:
Univ Nacl Autonoma Mexico, Dept Biol Mol & Biotecnol, Inst Invest Biomed, Mexico City 04510, Mexico
Agredano-Moreno, LT:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Mexico City 04510, Mexico
Jiménez-García, LF:
Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Mexico City 04510, Mexico
Ruiz-Ordaz, BH:
Univ Nacl Autonoma Mexico, Dept Biol Mol & Biotecnol, Inst Invest Biomed, Mexico City 04510, Mexico
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