A diet rich in omega-3 fatty acid improves periodontitis and tissue destruction by MMP2- and MMP9-linked inflammation in a murine model
Por:
González-Alva P., Solís-Suárez D.L., Cifuentes-Mendiola S.E., García-Hernández A.L.
Publicada:
1 ene 2024
Ahead of Print:
1 jun 2023
Categoría:
Dentistry (miscellaneous)
Resumen:
Periodontitis is an oral-cavity inflammatory disease and is the principal cause associated with tooth loss. Matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) are important proteases involved in periodontal tissue destruction. The omega-3 polyunsaturated fatty acids (?-3 PUFA) have been demonstrated to possess immunoregulatory properties in periodontitis. The aim of the study was to investigate the effects of ?-3 PUFA on inflammation and on the expression of MMP-2 and -9 in a murine periodontitis model. Twenty-four male C57BL/6 mice were divided into control mice (Control), control mice treated with ?-3 PUFA (O3), mice with periodontitis (P), and mice with periodontitis treated with ?-3 PUFA (P + O3). ?-3 PUFA were administered orally once a day for 70 days. Periodontitis in mice was induced by Porphyromonas gingivalis-infected ligature placement around the second maxillary molar. The mice were sacrificed, and blood and maxillary samples were collected. Flow cytometry was used to quantify tumor necrosis factor-alpha (TNFa), interleukin (IL)-2, IL-4, IL-5, and interferon-gamma. Histologic analysis and immunohistochemistry for MMP-2 and -9 were performed. The data were statistically evaluated using analysis of variance (ANOVA) and the Tukey post hoc test. Histological analysis showed that ?-3 PUFA supplementation prevented inflammation and tissue destruction and revealed that bone destruction was more extensive in the P group than in the P + O3 group (p < 0.05). Also, it decreased the serum expressions of TNFa and IL-2 and the tissue expression of MMP-2 and -9 in the periodontitis-induced model (p < 0.05). ?-3 PUFA supplementation prevented alveolar bone loss and periodontal destruction, probably by decreasing the expression of MMP-2 and MMP-9 and its immunoregulatory properties. © 2023, The Author(s).
Filiaciones:
Laboratory of Tissue Bioengineering, Faculty of Dentistry, National Autonomous University of Mexico, Mexico City, Mexico
Laboratory of Dental Research, Section of Osteoimmunology and Oral Immunology, FES Iztacala, National Autonomous University of Mexico, A. Jiménez Gallardo SN, San Sebastián Xhala, Cuautitlán Izcalli, 54714, Mexico
Postgraduate Course in Dental Sciences, National Autonomous University of Mexico, Mexico City, Mexico
hybrid, All Open Access; Hybrid Gold
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