Antibacterial and Cytotoxic Study of Hybrid Films Based on Polypropylene and NiO or NiFe2O4 Nanoparticles
Por:
Rincon-Granados K.L., Vázquez-Olmos A.R., Rodríguez-Hernández A.-P., Prado-Prone G., Rivera M., Garibay-Febles V., Almanza-Arjona Y.C., Sato-Berrú R.Y., Mata-Zamora E., Silva-Bermúdez P.S., Vega-Jiménez A.
Publicada:
1 ene 2023
Resumen:
This study presents an in vitro analysis of the bactericidal and cytotoxic properties of hybrid films containing nickel oxide (NiO) and nickel ferrite (NiFe2O4) nanoparticles embedded in polypropylene (PP). The solvent casting method was used to synthesize films of PP, PP@NiO, and PP@NiFe2O4, which were characterized by different spectroscopic and microscopic techniques. The X-ray diffraction (XRD) patterns confirmed that the small crystallite sizes of NiO and NiFe2O4 NPs were maintained even after they were incorporated into the PP matrix. From the Raman scattering spectroscopy data, it was evident that there was a significant interaction between the NPs and the PP matrix. Additionally, the Scanning Electron Microscopy (SEM) analysis revealed a homogeneous dispersion of NiO and NiFe2O4 NPs throughout the PP matrix. The incorporation of the NPs was observed to alter the surface roughness of the films; this behavior was studied by atomic force microscopy (AFM). The antibacterial properties of all films were evaluated against Pseudomonas aeruginosa (ATCC®: 43636™) and Staphylococcus aureus (ATCC®: 23235™), two opportunistic and nosocomial pathogens. The PP@NiO and PP@ NiFe2O4 films showed over 90% bacterial growth inhibition for both strains. Additionally, the effects of the films on human skin cells, such as epidermal keratinocytes and dermal fibroblasts, were evaluated for cytotoxicity. The PP, PP@NiO, and PP@NiFe2O4 films were nontoxic to human keratinocytes. Furthermore, compared to the PP film, improved biocompatibility of the PP@NiFe2O4 film with human fibroblasts was observed. The methodology utilized in this study allows for the production of hybrid films that can inhibit the growth of Gram-positive bacteria, such as S. aureus, and Gram-negative bacteria, such as P. aeruginosa. These films have potential as coating materials to prevent bacterial proliferation on surfaces. © 2023 by the authors.
Filiaciones:
Rincon-Granados K.L.:
Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México, 04510, Mexico
Vázquez-Olmos A.R.:
Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México, 04510, Mexico
Rodríguez-Hernández A.-P.:
Laboratorio de Genética Molecular, División de Estudios de Posgrado e Investigación de la Facultad de Odontología, Universidad Nacional Autónoma de México, Ciudad de México, 04510, Mexico
Prado-Prone G.:
Facultad de Odontología, División de Estudios de Posgrado e Investigación, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, Ciudad de México, 04510, Mexico
Rivera M.:
Instituto de Física, Universidad Nacional Autónoma de México, Ciudad de México, 04510, Mexico
Garibay-Febles V.:
Instituto Mexicano del Petróleo, Eje Central Lázaro Cárdenas Norte 152 Col. San Bartolo Atepehuacan, Ciudad de México, 07730, Mexico
Almanza-Arjona Y.C.:
Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Nuevo León, Monterrey, 64849, Mexico
Sato-Berrú R.Y.:
Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México, 04510, Mexico
Mata-Zamora E.:
Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad de México, 04510, Mexico
Silva-Bermúdez P.S.:
Unidad de Ingeniería de Tejidos, Terapia Celular y Medicina Regenerativa, Instituto Nacional de Rehabilitación Luis Guillermo Ibarra Ibarra, Ciudad de México, 14389, Mexico
Vega-Jiménez A.:
Facultad de Odontología, División de Estudios de Posgrado e Investigación, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, Ciudad de México, 04510, Mexico
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