Antimicrobial activity, viability, and physicochemical properties of an MTA-type cement with different concentrations of bismuth trioxide
Por:
Martinez-Hernandez M., Manzo-Garcia D., Ramirez-Ortega J.P., Arreguin-Cano J.A., Chavez-Cortez E.G., Arenas-Alatorre J.A., Rodriguez-Chavez J.A., Flores-Ledesma A.
Publicada:
1 ene 2023
Resumen:
In medicine, bismuth is used as an antimicrobial agent. In dentistry, it is used primarily to increase radiopacity in some endodontic materials. The objective is to evaluate the antimicrobial activity, cell viability, pH, solubility, film thickness, and setting time of a mineral trioxide aggregated (MTA)-types of cement with different concentrations of bismuth trioxide. Three experimental MTA-types of cement with a bismuth trioxide (Bi2O3) concentration of 15 wt%, 20 wt%, and 25 wt% were used. The antimicrobial activity test was conducted on Streptococcus mutans and Porphyromonas gingivalis strains. Cell viability was measured by the quantitative colorimetric assay using the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide) assay on a mouse fibroblast cell line (L929). Solubility, film thickness, and setting time were performed according to ISO 6876. The lowest Bi2O3 concentrations showed the best antimicrobial activity and cell viability. pH, solubility, setting time, and film thickness did not show statistically significant differences between the different Bi2O3 concentrations tested. © 2023 Miryam Martinez-Hernandez et al., published by Sciendo.
Filiaciones:
Biointerphases Laboratory, Studies, and Research Division. Faculty of Dentistry, National Autonomous University of Mexico, Circuito exterior s/n, Ciudad Universitaria04510 CDMX, Mexico
Dental Materials and Biomaterials Research Laboratory, Studies and Research Division. Faculty of Dentistry, National Autonomous University of Mexico, Circuito de la Investigacion Cientifica04510 CDMX, Mexico
Departement of Health of the State of Guerrero, Chilpancingo de Los Bravo, Guerrero, Guerrero, 39000, Mexico
School of Dentistry Mexicali, Autonomous University of Baja California, Mexicali, Baja California, 21040, Mexico
Central Microscopy Laboratory, Institute of Physics, National Autonomous University of México, Mexico City, Mexico
Department of Integral Dental Clinics, University Center for Health Sciences, University of Guadalajara, Guadalajara, Mexico
Dental Materials and Biomaterials Laboratory, Faculty of Stomatology, Meritorious Autonomous University of Puebla, Puebla, 72410, Mexico
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