Technical Implications of the Chicken Embryo Chorioallantoic Membrane Assay to Elucidate Neuroblastoma Biology
Por:
Patiño-Morales C.C., Jaime-Cruz R., Ramírez-Fuentes T.C., Villavicencio-Guzmán L., Salazar-García M.
Publicada:
1 ene 2023
Resumen:
The chorioallantoic membrane (CAM) can be used as a valuable research tool to examine tumors. The CAM can be used to investigate processes such as migration, invasion, and angiogenesis and to assess novel antitumor drugs. The CAM can be used to establish tumors in a straightforward, rapid, and cost-effective manner via xenotransplantation of cells or tumor tissues with reproducible results; furthermore, the use of the CAM adheres to the three “R” principle, i.e., replace, reduce, and refine. To achieve successful tumor establishment and survival, several technical aspects should be taken into consideration. The complexity and heterogeneity of diseases including neuroblastoma and cancers in general and their impact on human health highlight the importance of preclinical models that help us describe tumor-specific biological processes. These models will not only help in understanding tumor biology, but also allow clinicians to explore therapeutic alternatives that will improve current treatment strategies. In this review, we summarize the technical characteristics as well as the main findings regarding the use of this model to study neuroblastoma for angiogenesis, metastasis, drug sensitivity, and drug resistance. © 2023 by the authors.
Filiaciones:
Patiño-Morales C.C.:
Developmental Biology Research Laboratory, Hospital Infantil de México Federico Gómez, Mexico City, 06720, Mexico
Cell Biology Laboratory, Universidad Autónoma Metropolitana-Cuajimalpa, Mexico City, 05348, Mexico
Jaime-Cruz R.:
Developmental Biology Research Laboratory, Hospital Infantil de México Federico Gómez, Mexico City, 06720, Mexico
Department of Health Sciences, Universidad Tecnológica de México-UNITEC México-Campus Sur, Mexico City, 09810, Mexico
Ramírez-Fuentes T.C.:
Developmental Biology Research Laboratory, Hospital Infantil de México Federico Gómez, Mexico City, 06720, Mexico
Section of Graduate Studies and Research, School of Medicine of the National Polytechnic Institute, Mexico City, 11340, Mexico
Villavicencio-Guzmán L.:
Developmental Biology Research Laboratory, Hospital Infantil de México Federico Gómez, Mexico City, 06720, Mexico
Salazar-García M.:
Developmental Biology Research Laboratory, Hospital Infantil de México Federico Gómez, Mexico City, 06720, Mexico
Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, 04360, Mexico
Green Published, gold, All Open Access; Gold
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