The conducting state of TRPA1 modulates channel lateral mobility
Por:
Sampieri A., Padilla-Flores T., Thawani A.R., Lam P.-Y., Fuchter M.J., Peterson R., Vaca L.
Publicada:
1 dic 2023
Ahead of Print:
1 sep 2023
Resumen:
We have studied Danio rerio (Zebrafish) TRPA1 channel using a method that combines single channel electrophysiological and optical recordings to evaluate lateral mobility and channel gating simultaneously in single channels. TRPA1 channel activation by two distinct chemical ligands: allyl isothiocyanate (AITC) and TRPswitch B, results in substantial reduction of channel lateral mobility at the plasma membrane. Incubation with the cholesterol sequestering agent methyl-beta-cyclodextrin (M beta CD), prevents the reduction on lateral mobility induced by the two chemical agonists. This results strongly suggest that the open conformation of TRPA1 modulates channel lateral mobility probably by facilitating the insertion of the channel into cholesterol-enriched domains at the plasma membrane.
Filiaciones:
Sampieri A.:
Instituto de Fisiología Celular. Departamento de Biología Celular y del desarrollo. Universidad Nacional Autónoma de México. México, CDMX04510, Mexico
Padilla-Flores T.:
Instituto de Fisiología Celular. Departamento de Biología Celular y del desarrollo. Universidad Nacional Autónoma de México. México, CDMX04510, Mexico
Thawani A.R.:
Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, White City Campus, London, W12 OBZ, United Kingdom
Lam P.-Y.:
Department of Cell Biology, Neurobiology and Anatomy, Medical College of Wisconsin, 8701 West Watertown Plank Rd., Milwaukee, WI 53226, United States
Neuroscience Research Center, Medical College of Wisconsin, 8701 West Watertown Plank Rd., Milwaukee, WI 53226, United States
Fuchter M.J.:
Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, White City Campus, London, W12 OBZ, United Kingdom
Peterson R.:
College of Pharmacy, University of Utah, 30 South 2000 East, Salt Lake City, UT 84112, United States
Vaca L.:
Instituto de Fisiología Celular. Departamento de Biología Celular y del desarrollo. Universidad Nacional Autónoma de México. México, CDMX04510, Mexico
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