Microbacterium plantarum sp. nov. and Microbacterium thalli sp. nov., two endophytic metal-resistant bacteria isolated from Sphaeralcea angustifolia (Cav.) G. Don and Prosopis laevigata (Humb. et Bonpl. ex Willd) M.C. Johnston
Por:
Arroyo-Herrera I., Román-Ponce B., Bustamante-Brito R., Guevara-Luna J., Larios-Serrato V., Carro L., Igual J.M., Geiger O., Sánchez-Reyes A., Santos P.E., Wang E.T., Vásquez-Murrieta M.S.
Publicada:
1 ene 2023
Resumen:
Four Gram-positive, aerobic, catalase-and oxidase-negative, rod-shaped, motile endophytic bacterial strains, designated NM3R9T, NE1TT3, NE2TL11 and NE2HP2T, were isolated from the inner tissues (leaf and stem) of Sphaeralcea angustifolia and roots of Prosopis laevigata. They were characterized using a polyphasic approach, which revealed that they represent two novel Microbacterium species. Phylogenetic analysis based on 16S rRNA gene sequencing showed that the species closest to NE2HP2T was Microbacterium arborescens DSM 20754T (99.6%) and that closest to NM3R9T, NE2TL11 and NE2TT3 was Microbacterium oleivorans NBRC 103075T (97.4%). The whole-genome average nucleotide identity value between strain NM3R9T and Microbacterium imperiale DSM 20530T was 90.91%, and that between strain NE2HP2T and M. arborecens DSM 20754T was 91.03%. Digital DNA–DNA hybridization showed values of less than 70% with the type strains of related species. The polar lipids present in both strains included diphosphatidylglycerol, phosphatidylglycerol, glycolipids and unidentified lipids, whereas the major fatty acids included anteiso-C15: 0, anteiso-C17: 0, iso-C16: 0 and C16: 0. Whole-cell sugars included mannose, rhamnose and galactose. Strains NM3R9T and NE2HP2T showed physiological characteristics different from those present in closely related Microbacterium species. According to the taxonomic analysis, both strains belong to two novel species. The name Microbacterium plantarum sp. nov. is proposed for strain NE2HP2T (=LMG 30875T=CCBAU 101117T) and Microbacterium thalli sp. nov. for strains NM3R9T (=LMG 30873T=CCBAU 101116T), NE1TT3 (=CCBAU 101114) and NE2TL11 (=CCBAU 101115). © 2023, Microbiology Society. All rights reserved.
Filiaciones:
Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del Miguel Hidalgo, Mexico City, 11340, Mexico
Universidad Politécnica del Estado de Morelos, Boulevard Cuauhnáhuac #566, Col. Lomas del Texcal, Morelos, Jiutepec, 62550, Mexico
Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad s/n, Colonia Chamilpa, Morelos, Cuernavaca, 62210, Mexico
Departamento de Microbiología y Genética, Facultad de CC Agrarias y Ambientales, Universidad de Salamanca, Plaza Doctores de la Reina, Lab 230, Salamanca, 37007, Spain
Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), c/Cordel de Merinas 40-52, Salamanca, 37008, Spain
Investigador por México, CONAHCYT-Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Colonia Chamilpa, Morelos, Cuernavaca, 62209, Mexico
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