Functional annotation and comparative genomics analysis of Balamuthia mandrillaris reveals potential virulence-related genes
Por:
Otero-Ruiz, Alejandro, Rodriguez-Anaya, Libia Zulema, Lares-Villa, Fernando, Lozano Aguirre Beltrán L.F., Lares-Jimenez, Luis Fernando, Gonzalez-Galaviz, Jose Reyes, Cruz-Mendivil, Abraham
Publicada:
31 ago 2023
Categoría:
Multidisciplinary
Resumen:
Balamuthia mandrillaris is a pathogenic protozoan that causes a rare but
almost always fatal infection of the central nervous system and, in some
cases, cutaneous lesions. Currently, the genomic data for this
free-living amoeba include the description of several complete
mitochondrial genomes. In contrast, two complete genomes with draft
quality are available in GenBank, but none of these have a functional
annotation. In the present study, the complete genome of B. mandrillaris
isolated from a freshwater artificial lagoon was sequenced and
assembled, obtaining an assembled genome with better assembly quality
parameter values than the currently available genomes. Afterward, the
genome mentioned earlier, along with strains V039 and 2046, were
subjected to functional annotation. Finally, comparative genomics
analysis was performed, and it was found that homologous genes in the
core genome potentially involved in the virulence of Acanthamoeba spp.
and Trypanosoma cruzi. Moreover, eleven of fifteen genes were identified
in the three strains described as potential target genes to develop new
treatment approaches for B. mandrillaris infections. These results
describe proteins in this protozoan's complete genome and help
prioritize which target genes could be used to develop new treatments.
Filiaciones:
Otero-Ruiz, Alejandro:
Programa de Doctorado en Ciencias Especialidad en Biotecnología, Departamento de Biotecnología y Ciencias Alimentarias, Instituto Tecnológico de Sonora, Ciudad Obregón, Sonora 85000, Mexico
Rodriguez-Anaya, Libia Zulema:
CONAHCYT-Instituto Tecnológico de Sonora, Ciudad Obregón, Sonora 85000, Mexico
Lares-Villa, Fernando:
Departamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, Ciudad Obregón, Sonora 85000, Mexico
Lozano Aguirre Beltrán L.F.:
Unidad de Análisis Bioinformáticos, Centro de Ciencias Genómicas de la Universidad Nacional Autónoma de México (UNAM), Cuernavaca, Morelos 62210, Mexico
Lares-Jimenez, Luis Fernando:
Departamento de Ciencias Agronómicas y Veterinarias, Instituto Tecnológico de Sonora, Ciudad Obregón, Sonora 85000, Mexico
Gonzalez-Galaviz, Jose Reyes:
CONAHCYT-Instituto Tecnológico de Sonora, Ciudad Obregón, Sonora 85000, Mexico
Cruz-Mendivil, Abraham:
CONAHCYT-Instituto Politécnico Nacional, CIIDIR Unidad Sinaloa, Guasave, Sinaloa 81101, Mexico
gold, All Open Access; Gold
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