A molecular and ecological study of Macracanthorhynchus ingens (von Linstow, 1879) (Acanthocephala: Archiacanthocephala), in its paratenic and definitive hosts in southeastern Mexico and the Eastern USA
Por:
Ortega-Olivares, Mirza Patricia, Velazquez-Urrieta, Yanet, Sereno-Uribe, Ana Lucia, Harvey, Michael B., Garcia-Varela, Martin
Publicada:
1 oct 2023
Ahead of Print:
1 jun 2023
Categoría:
Parasitology
Resumen:
The acanthocephalan Macracanthorhynchus ingens (von Linstow 1879)
(Acanthocephala: Archiacanthocephala) is a parasite that infects the gut
of carnivores (racoons, coyotes, wolves, foxes, badgers, skunks,
opossum, mink and bears) as an adult and the body cavity of lizards,
snakes, and frogs as a cystacanth in the Americas. In this study, adults
and cystacanths of M. ingens from southeastern Mexico and southern
Florida, USA, were identified morphologically by having a cylindrical
proboscis armed with 6 rows of hooks each with 6 hooks. Hologenophores
were used to sequence the small (SSU) and large (LSU) subunits of
ribosomal DNA and cytochrome c oxidase subunit 1 (cox 1) from
mitochondrial DNA. Phylogenetic analysis of the new SSU and LSU
sequences of M. ingens placed them in a clade with other sequences
available in GenBank identified as M. ingens. The cox 1 tree showed that
the nine new sequences and six previously published sequences of M.
ingens from the USA form a clade with other sequences previously
identified as M. ingens from GenBank. The intraspecific genetic
divergence among isolates from the Americas ranged from 0 to 2%, and in
combination with the phylogenetic trees confirmed that the isolates
belonged to the same species. The cox 1 haplotype network inferred with
15 sequences revealed 10 haplotypes separated from each other by a few
substitutions. Rio Grande Leopard Frogs and Vaillant & PRIME;s Frogs
harbored cystacanths with low prevalence, 28% and 37% respectively, in
Mexico. Brown Basilisks, an invasive lizard in Florida, USA, had high
values of prevalence, 92% and 93% in males and females, respectively.
Females harbored more cystacanths than males (0-39 vs 0-21) for unknown
reasons that may, however, be related to ecological differences.
Filiaciones:
Ortega-Olivares, Mirza Patricia:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Ciudad Univ, Mexico City 04510, Mexico
Velazquez-Urrieta, Yanet:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Ciudad Univ, Mexico City 04510, Mexico
Sereno-Uribe, Ana Lucia:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Ciudad Univ, Mexico City 04510, Mexico
Harvey, Michael B.:
Broward Coll, Dept Biol Sci, 3501 SW Davie Rd, Davie, FL USA
Garcia-Varela, Martin:
Univ Nacl Autonoma Mexico, Dept Zool, Inst Biol, Ciudad Univ, Mexico City 04510, Mexico
hybrid
|