Standardizing an Experimental Murine Model of Extraparenchymal Neurocysticercosis That Immunologically Resembles Human Infection
Por:
Espinosa-Ceron, Alejandro, Mendez, Alejandro, Hernandez-Aceves, Juan, Juarez-Gonzalez, Juan C., Villalobos, Nelly, Hernandez, Marisela, Diaz, Georgina, Soto, Paola, Concha, Luis, Perez-Osorio, Ivan N., Ortiz-Retana, Juan J., Bobes, Raul J., Parkhouse, Robert M., Hamamoto, P. T., Fragoso, Gladis, Sciutto, Edda
Publicada:
1 jul 2023
Categoría:
Neuroscience (miscellaneous)
Resumen:
Background: Neurocysticercosis (NCC) is endemic in non-developed regions
of the world. Two forms of NCC have been described, for which
neurological morbidity depends on the location of the lesion, which can
be either within the cerebral parenchyma or in extraparenchymal spaces.
The extraparenchymal form (EXP-NCC) is considered the most severe form
of NCC. EXP-NCC often requires several cycles of cysticidal treatment
and the concomitant use of glucocorticoids to prevent increased
inflammation, which could lead to intracranial hypertension and, in rare
cases, to death. Thus, the improvement of EXP-NCC treatment is greatly
needed. Methods: An experimental murine model of EXP-NCC, as an adequate
model to evaluate new therapeutic approaches, and the parameters that
support it are described. EXP-NCC was established by injecting 30 Taenia
crassiceps cysticerci, which are less than 0.5 mm in diameter, into the
cisterna magna of male and female Wistar rats. Results: Cyst
implantation and infection progression were monitored by detecting the
HP10 antigen and anti-cysticercal antibodies in the serum and cerebral
spinal fluid (CSF) of infected rats and by magnetic resonance imaging.
Higher HP10 levels were observed in CSF than in the sera, as in the case
of human EXP-NCC. Low cell recruitment levels were observed surrounding
established cysticerci in histological analysis, with a modest increase
in GFAP and Iba1 expression in the parenchyma of female animals. Low
cellularity in CSF and low levels of C-reactive protein are consistent
with a weak inflammatory response to this infection. After 150 days of
infection, EXP-NCC is accompanied by reduced levels of mononuclear cell
proliferation, resembling the human disease. EXP-NCC does not affect the
behavior or general status of the rats. Conclusions: This model will
allow the evaluation of new approaches to control neuroinflammation and
immunomodulatory treatments to restore and improve the specific
anti-cysticercal immunity in EXP-NCC.
Filiaciones:
Espinosa-Ceron, Alejandro:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
Mendez, Alejandro:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
Hernandez-Aceves, Juan:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
Juarez-Gonzalez, Juan C.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
Villalobos, Nelly:
Univ Nacl Autonoma Mexico, Fac Med Vet & Zootecnia, Ciudad De Mexico 04510, Mexico
Hernandez, Marisela:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
Diaz, Georgina:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
Soto, Paola:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
Concha, Luis:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Queretaro 76230, Mexico
Perez-Osorio, Ivan N.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
Ortiz-Retana, Juan J.:
Univ Nacl Autonoma Mexico, Inst Neurobiol, Queretaro 76230, Mexico
Bobes, Raul J.:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
Parkhouse, Robert M.:
Inst Gulbekian Ciencia, Portugal R Qta Grande 6, P-2780156 Oeiras, Portugal
Hamamoto, P. T.:
UNESP Univ Estadual Paulista, Botucatu Med Sch, Dept Neurol Psychol & Psychiat, BR-18618687 Sao Paulo, Brazil
Fragoso, Gladis:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
Sciutto, Edda:
Univ Nacl Autonoma Mexico, Inst Invest Biomed, Ciudad de Mexico 04510, Mexico
gold, All Open Access; Gold
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