Antifungal nanocomposites inspired by titanate nanotubes for complete inactivation of botrytis cinerea isolated from tomato infection
Por:
Rodríguez-González V., Domínguez-Espíndola R.B., Casas-Flores S., Patrón-Soberano O.A., Camposeco-Solis R., Lee S.-W.
Publicada:
1 ene 2016
Resumen:
Antifungal silver nanocomposites inspired by titanate nanotubes (AgTNTs) were successfully evaluated for the effective inactivation of the phytopathogenic fungus Botrytis cinerea within 20 min. One-dimensional H2Ti3O7 nanotubes functionalized with silver nanoparticles (AgNPs) exhibit unique surface and antifungal properties for the photoinactivation of B. cinerea. Nanostructured titanates were synthesized by the eco-friendly, practical, microwave-induced, hydrothermal method followed by a highly monodispersive AgNP UV-photodeposition. Protonated nanotubes of ~11 nm in diameter and four-layers displayed high surface areas, 300 m2/g, with a size functionalization of 5 nm for the AgNPs. UV-vis DRS and XPS allowed the characterization and/or quantification of surface reactive species and cytotoxic silver species such as Ag°, Ag+. The effective biocidal properties of the nanocomposites were confirmed by using the well-known Gram-negative bacteria Escherichia coli, and then proceeding to the effective inactivation of the phytopathogenic fungus under visible light. The photoassisted inactivation mechanism was examined by HAADF-STEM, HRTEM, and FESEM electronic microscopies. A plasmalemma invagination due to oxidative stress caused by reactive oxygen, silver cytotoxicity species, and AgTNT sharp morphology damage expands the conidia to induce the cell death. The impact of the eco-friendly inactivation is significant because of the ease with which it is carried out and the possibility of being performed in situ with plants like tomato and grapes, which are ranked among the most valuable agricultural products worldwide. © 2016 American Chemical Society.
Filiaciones:
División de Materiales Avanzados, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, San Luis Potosí, S.L.P., CP 78216, Mexico
Posgrado en Ingeniería y Ciencias Aplicadas, Universidad Autónoma Del Estado de Morelos, Av. Universidad # 1001, Col. Chamilpa, Cuernavaca, Morelos, C.P. 62209, Mexico
División de Biología Molecular, Instituto Potosino de Investigación Científica y Tecnológica, Camino a la Presa San José 2055, San Luis Potosí, S.L.P., CP 78216, Mexico
Global Research Laboratory, Sun Moon University, Galsan-Ri, Tangjung-Myon, Asan Chungnam, 336-708, South Korea
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