In Vitro and In Vivo Cysticidal Effects of Carica Papaya Cell Suspensions
Por:
Guzmán C., Villalobos N., Ortiz Caltempa A., Hernández M., Núñez G., Salazar J., Bobes R.J., Fragoso G., Sciutto E., Villarreal M.L.
Publicada:
1 ene 2023
Resumen:
Parasitic diseases are a major public health problem worldwide. Plant-derived products appear to be ideal candidates from a biotechnological perspective, being sustainable and environmentally friendly. The antiparasitic properties of Carica papaya have been attributed to some of its components, including papain and other compounds that are concentrated in the latex and seeds. This study demonstrated in vitro a high and insignificantly different cysticidal activity of soluble extract that was obtained after the disruption of nontransformed wild-type (WT) cells as well as transformed papaya calluses (PC-9, PC-12, and PC-23) and papaya cell suspensions (CS-9, CS-12, and CS-23). In vivo, cell suspensions of CS-WT and CS-23 that had been previously lyophilized were tested with respect to their cysticidal effects, compared with those of three commercial antiparasitic drugs. CS-WT and CS-23 together reduced the number of cysticerci, the number of buds, and the percentage of calcified cysticerci in a similar extent to albendazole and niclosamide, whereas ivermectin was less effective. Mice were then orally immunized with CS-23 that expressed the anti-cysticercal KETc7 antigen (10 µg/mouse), CS-WT (10 mg/mouse), or both together to evaluate their preventive properties. CS-23 and CS-WT significantly reduced the expected parasite and increased the percentage of calcified cysticerci as well as recovery, being more effective when employed together. The results reported in this study support the feasibility of the development of an anti-cysticercosis vaccine from cells of C. papaya in in vitro cultures, as they are a source of an anthelmintic, natural, and reproducible product.
Filiaciones:
Guzmán C.:
Biotechnology Research Center, Autonomous University of the State of Morelos, Cuernavaca, Morelos, Mexico
Department of Immunology, Instituto de Investigaciones Biomédicas. Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Villalobos N.:
Department of Pathology, Faculty of Veterinary Medicine and Zootechnics, National Autonomous University of Mexico, Ciudad Universitaria. Alcaldia CoyoacanMexico City, Mexico
Ortiz Caltempa A.:
Biotechnology Research Center, Autonomous University of the State of Morelos, Cuernavaca, Morelos, Mexico
Hernández M.:
Department of Immunology, Instituto de Investigaciones Biomédicas. Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Núñez G.:
Faculty of Veterinary Medicine and Zootechnics, Academic Unit No. 2, Autonomous University of Guerrero, Cuajinicuilapa, Guerrero, Mexico
Salazar J.:
Research Direction, La Salle UniversityMexico City, Mexico
Bobes R.J.:
Department of Immunology, Instituto de Investigaciones Biomédicas. Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Fragoso G.:
Department of Immunology, Instituto de Investigaciones Biomédicas. Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Sciutto E.:
Department of Immunology, Instituto de Investigaciones Biomédicas. Universidad Nacional Autónoma de México, Ciudad de México, Mexico
Villarreal M.L.:
Biotechnology Research Center, Autonomous University of the State of Morelos, Cuernavaca, Morelos, Mexico
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