Clinical validation of 3D-printed swabs in adults and children for SARS-CoV-2 detection
Por:
Sanchez-Sandoval A.L., Sánchez-Pérez C., García-García J.A., Uriega-González S.P., Guerrero-Avendaño G.M.L., Barrón-Palma E.V.
Publicada:
1 ene 2023
Resumen:
Throughout the entire coronavirus disease 19 (COVID-19) pandemic, there were disruptions in the supply chain of test materials around the world, primarily in poor- and middle-income countries. The use of 3D prints is an alternative to address swab supply shortages. In this study, the feasibility of the clinical use of 3D-printed swabs for oropharyngeal and nasopharyngeal sampling for the detection of SARS-CoV-2 infection was evaluated. For that purpose, paired samples with the 3D printed and the control swabs were taken from 42 adult patients and 10 pediatric patients, and the results obtained in the detection of SARS-CoV-2 by reverse transcription and quantitative polymerase chain reaction (RT-qPCR) were compared. Additionally, in those cases where the result was positive for SARS-CoV-2, the viral load was calculated by means of a mathematical algorithm proposed by us. For both adults and children, satisfactory results were obtained in the detection of SARS-CoV-2 by RT-qPCR; no significant differences were found in the quantification cycle values between the 3D-printed swab samples and the control samples. Furthermore, we corroborated that the 3D-printed swabs caused less discomfort and pain at the time of sampling. In conclusion, this study shows the feasibility of routinely using 3D-printed swabs for both adults and children. In this way, it is possible to maintain local and cheaper consumption along with fewer distribution difficulties. © 2023 The Author(s).
Filiaciones:
Sanchez-Sandoval A.L.:
Servicio de Medicina Genómica, Hospital General de México "Dr. Eduardo Liceaga", Mexico City, Mexico
Sánchez-Pérez C.:
Instituto de Ciencias Aplicadas y Tecnología, Universidad Nacional Autónoma de México, Mexico City, Mexico
García-García J.A.:
Dirección de Educación y Capacitación en Salud. Hospital General de México "Dr. Eduardo Liceaga", Mexico City, Mexico
Uriega-González S.P.:
Servicio de Pediatría, Hospital General de México "Dr. Eduardo Liceaga", Mexico City, Mexico
Guerrero-Avendaño G.M.L.:
Dirección General, Hospital General de México "Dr. Eduardo Liceaga", Mexico City, Mexico
Barrón-Palma E.V.:
Servicio de Medicina Genómica, Hospital General de México "Dr. Eduardo Liceaga", Mexico City, Mexico
All Open Access, Gold
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