Zearalenone Does Not Show Genotoxic Effects in the Drosophila melanogaster Wing Spot Test, but It Induces Oxidative Imbalance, Development, and Fecundity Alterations
Por:
Santos-Cruz, Luis Felipe, Ponciano-Gomez, Alberto, Torres-Gregorio, Juan Tomas, Ramirez-Cruz, Bertha Guadalupe, Vazquez-Gomez, Gerardo, Hernandez-Portilla, Luis Barbo, Flores-Ortiz, Cesar Mateo, Duenas-Garcia, Irma Elena, Heres-Pulido, Maria Eugenia, Castaneda-Partida, Laura, Duran-Diaz, Angel, Campos-Aguilar, Myriam, Sigrist-Flores, Santiago Cristobal, Piedra-Ibarra, Elias
Publicada:
1 jun 2023
Resumen:
Zearalenone (ZEN) is a non-steroidal mycoestrogen produced by the
Fusarium genus. ZEN and its metabolites compete with 17-beta estradiol
for cytosolic estrogen receptors, causing reproductive alterations in
vertebrates. ZEN has also been associated with toxic and genotoxic
effects, as well as an increased risk for endometrial adenocarcinomas or
hyperplasia, breast cancer, and oxidative damage, although the
underlying mechanisms remain unclear. Previous studies have monitored
cellular processes through levels of transcripts associated with Phase I
Xenobiotic Metabolism (Cyp6g1 and Cyp6a2), oxidative stress (hsp60 and
hsp70), apoptosis (hid, grim, and reaper), and DNA damage genes (Dmp53).
In this study, we evaluated the survival and genotoxicity of ZEN, as
well as its effects on emergence rate and fecundity in Drosophila
melanogaster. Additionally, we determined levels of reactive oxygen
species (ROS) using the D. melanogaster flare and Oregon R(R)-flare
strains, which differ in levels of Cyp450 gene expression. Our results
showed that ZEN toxicity did not increase mortality by more than 30%.
We tested three ZEN concentrations (100, 200, and 400 & mu;M) and found
that none of the concentrations were genotoxic but were cytotoxic.
Taking into account that it has previously been demonstrated that ZEN
administration increased hsp60 expression levels and apoptosis gene
transcripts in both strains, the data agree with an increase in ROS and
development and fecundity alterations. Since Drosophila lacks homologous
genes for mammalian estrogen receptors alpha and beta, the effects of
this mycotoxin can be explained by a mechanism different from estrogenic
activity.
Filiaciones:
Santos-Cruz, Luis Felipe:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Toxicol Genet, Biol, Barrios 1, Tlalnepantla 54090, Mexico
Ponciano-Gomez, Alberto:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Lab Inmunol UMF, Barrios 1, Tlalnepantla 54090, Mexico
Torres-Gregorio, Juan Tomas:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Fisiol Vegetal UBIPRO, Barrios 1, Tlalnepantla 54090, Mexico
Ramirez-Cruz, Bertha Guadalupe:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Toxicol Genet, Biol, Barrios 1, Tlalnepantla 54090, Mexico
Vazquez-Gomez, Gerardo:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Fisiol Vegetal UBIPRO, Barrios 1, Tlalnepantla 54090, Mexico
Czech Acad Sci, Dept Cytokinet, Inst Biophys, Brno 61265, Czech Republic
Hernandez-Portilla, Luis Barbo:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Fisiol Vegetal UBIPRO, Barrios 1, Tlalnepantla 54090, Mexico
Flores-Ortiz, Cesar Mateo:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Fisiol Vegetal UBIPRO, Barrios 1, Tlalnepantla 54090, Mexico
Duenas-Garcia, Irma Elena:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Toxicol Genet, Biol, Barrios 1, Tlalnepantla 54090, Mexico
Heres-Pulido, Maria Eugenia:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Toxicol Genet, Biol, Barrios 1, Tlalnepantla 54090, Mexico
Castaneda-Partida, Laura:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Toxicol Genet, Biol, Barrios 1, Tlalnepantla 54090, Mexico
Duran-Diaz, Angel:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Math, Barrios 1, Tlalnepantla 54090, Mexico
Campos-Aguilar, Myriam:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Lab Inmunol UMF, Barrios 1, Tlalnepantla 54090, Mexico
Sigrist-Flores, Santiago Cristobal:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Lab Inmunol UMF, Barrios 1, Tlalnepantla 54090, Mexico
Piedra-Ibarra, Elias:
Univ Nacl Autonoma Mexico, Fac Estudios Super Iztacala, Fisiol Vegetal UBIPRO, Barrios 1, Tlalnepantla 54090, Mexico
gold, Green Published, All Open Access, Gold, Green
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