Advances in tissue engineering of cancer microenvironment-from three-dimensional culture to three-dimensional printing
Por:
Marques, Joana Rita Oliveira Faria, Gonzalez-Alva, Patricia, Lin, Ruby Yu-Tong, Fernandes, Beatriz Ferreira, Chaurasia, Akhilanand, Dubey, Nileshkumar
Publicada:
1 jun 2023
Ahead of Print:
1 jun 2023
Resumen:
Cancer treatment development is a complex process, with tumor
heterogeneity and inter-patient variations lim-iting the success of
therapeutic intervention. Traditional two-dimensional cell culture has
been used to study cancer metabolism, but it fails to capture
physiologically relevant cell-cell and cell-environment interactions
re-quired to mimic tumor-specific architecture. Over the past three
decades, research efforts in the field of 3D cancer model fabrication
using tissue engineering have addressed this unmet need. The
self-organized and scaffold-based model has shown potential to study the
cancer microenvironment and eventually bridge the gap between 2D cell
culture and animal models. Recently, three-dimensional (3D) bioprinting
has emerged as an exciting and novel biofabrication strategy aimed at
developing a 3D compartmentalized hierarchical organization with the
precise positioning of biomolecules, including living cells. In this
review, we discuss the advancements in 3D culture techniques for the
fabrication of cancer models, as well as their benefits and limitations.
We also highlight fu-ture directions associated with technological
advances, detailed applicative research, patient compliance, and
regulatory challenges to achieve a successful bed-to-bench transition.
Filiaciones:
Gonzalez-Alva, Patricia:
Natl Autonomous Univ Mexico UNAM, Postgrad Studies & Res Div, Tissue Bioengn Lab, Fac Dent, Mexico City 04510, Mexico
Lin, Ruby Yu-Tong:
Natl Univ Singapore, Fac Dent, Singapore, Singapore
Fernandes, Beatriz Ferreira:
Univ Lisbon, Fac Med Dent, Unidade Invest Ciencias Orais & Biomed UICOB, Oral Biol & Biochem Res Grp GIBBO, Lisbon, Portugal
Chaurasia, Akhilanand:
King Georges Med Univ, Fac Dent Sci, Dept Oral Med, Lucknow, Uttar Pradesh, India
Dubey, Nileshkumar:
Natl Univ Singapore, Fac Dent, Singapore, Singapore
Natl Univ Singapore, ORCHIDS Oral Care Hlth Innovat & Designs Singapor, Singapore, Singapore
gold, All Open Access; Gold
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