Molecular detection of Wolbachia and Bartonella as part of the microbiome of phlebotomine sand flies from Chiapas, Mexico
Por:
Lozano-Sardaneta, Yokomi N. N., Marina, Carlos F. F., Torres-Monzon, Jorge A. A., Sanchez-Cordero, Victor, Becker, Ingeborg
Publicada:
1 ene 2023
Ahead of Print:
1 abr 2023
Resumen:
Phlebotomine sand flies are dipterans of relevance due to their role as vectors of several pathogens worldwide. Bacteria in the gut of sand flies possibly affect their vectorial capacity and competence to transmit parasites. A retrospective study was performed in sand fly specimens that had previously been collected in four localities of the state of Chiapas during the period 2009–2011 to detect Wolbachia and Bartonella and their possible coinfection with Leishmania. For the molecular detection of bacteria, we used primers and conditions that had previously been reported. A total of 531 sand fly specimens of 10 species were analyzed. Four Wolbachia strains were detected in five sand fly species, showing a prevalence of 8.6%. All the Wolbachia strains had previously been reported in other taxa. In one sand fly species, we also detected a new lineage of Bartonella evidenced by a phylogenetic analysis. No sand fly specimens showed coinfections of these bacteria and Leishmania. The bacteria found in the phlebotomine sand flies are possibly transmitted by plant-mediated horizontal transmission and during blood meal feeding. © 2023, The Author(s).
Filiaciones:
Centro de Medicina Tropical, Unidad de Medicina Experimental, Facultad de Medicina de la Universidad Nacional Autónoma de México, Hospital General de México, Dr. Balmis 148, Col. Doctores, Mexico City, 06726, Mexico
Centro Regional de Investigación en Salud Pública, Instituto Nacional de Salud Pública (CRISP-INSP), Chiapas, Tapachula, Mexico
Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad de México, 04510, Mexico
Univ Nacl Autonoma Mexico, Hosp Gen Mexico, Ctr Med Trop, Unidad Med Expt,Fac Med, Dr Balmis 148, Mexico City 06726, DF, Mexico
Inst Nacl Salud Publ CRISP INSP, Ctr Reg Invest Salud Publ, Tapachula, Chiapas, Mexico
Univ Nacl Autonoma Mexico, Inst Biol, Mexico City 04510, DF, Mexico
hybrid, All Open Access; Hybrid Gold
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