Virulence Factors of the Gut Microbiome Are Associated with BMI and Metabolic Blood Parameters in Children with Obesity
Por:
Murga-Garrido, S. M., Ulloa-Perez, E. J., Diaz-Benitez, C. E., Orbe-Orihuela, Y. C., Cornejo-Granados, F., Ochoa-Leyva, A., Sanchez-Flores, A., Cruz, M., Castaneda-Marquez, A. C., Plett-Torres, T., Burguete Garcia, A. I., Lagunas-Martinez, A.
Publicada:
1 ene 2023
Ahead of Print:
1 feb 2023
Resumen:
The alteration of gut microbiome composition has been commonly observed
in diseases involving inflammation, such as obesity and metabolic
impairment. Inflammatory host response in the gut can be a consequence
of dietary driven dysbiosis.
The development of metabolic diseases is linked to the gut microbiota. A
cross-sectional study involving 45 children (6 to 12 years old) was
conducted to investigate the relationship between gut microbiota and
childhood obesity. Anthropometric and metabolic measurements,
food-frequency questionnaires (FFQs), and feces samples were obtained.
Using the body mass index (BMI) z-score, we categorized each participant
as normal weight (NW), or overweight and obese (OWOB). We determined 2
dietary profiles: one with complex carbohydrates and proteins (pattern
1), and the other with saturated fat and simple carbohydrates (pattern
2). The microbial taxonomic diversity and metabolic capacity were
determined using shotgun metagenomics. We found differences between both
BMI groups diversity. Taxa contributing to this difference, included
Eubacterium sp., Faecalibacterium prausnitzii, Dialister, Monoglobus
pectinilyticus, Bifidobacterium pseudocatenulatum, Intestinibacter
bartlettii, Bacteroides intestinalis, Bacteroides uniformis, and
Methanobrevibacter smithii. Metabolic capacity differences found between
NW and OWOB, included the amino acid biosynthesis pathway, the cofactor,
carrier, and vitamin biosynthesis pathway, the nucleoside and nucleotide
biosynthesis and degradation pathways, the carbohydrate-sugar
degradation pathway, and the amine and polyamine biosynthesis pathway.
We found significant associations between taxa such as Ruminococcus,
Mitsuokella multacida, Klebsiella variicola, and Citrobacter spp.,
metabolic pathways with the anthropometric, metabolic, and dietary data.
We also found the microbiome's lipooligosaccharide (LOS) category as
differentially abundant between BMI groups. Metabolic variations emerge
during childhood as a result of complex nutritional and microbial
interactions, which should be explained in order to prevent metabolic
illnesses in adolescence and maturity.IMPORTANCE The alteration of gut
microbiome composition has been commonly observed in diseases involving
inflammation, such as obesity and metabolic impairment. Inflammatory
host response in the gut can be a consequence of dietary driven
dysbiosis. This response is conducive to blooms of particular bacterial
species, adequate to survive in an inflammatory environment by means of
genetical capability of utilizing alternative nutrients. Understanding
the genomic and metabolic contribution of microbiota to inflammation,
including virulence factor prevalence and functional potential, will
contribute to identifying modifiable early life exposures and preventive
strategies associated with obesity risk in childhood.
Filiaciones:
Inst Nacl Salud Publ, Ctr Invest Enfermedades Infecciosas, Cuernavaca, Mexico
Univ Nacl Autonoma Mexico, Fac Med, PECEM, Mexico City, Mexico
Univ Washington, Dept Biostat, Seattle, WA USA
Univ Nacl Autonoma Mexico, Dept Microbiol Mol, Inst Biotecnol, Cuernavaca, Mexico
Univ Nacl Autonoma Mexico, Unidad Univ Secuenciac Mas & Bioinformat, Inst Biotecnol, Cuernavaca, Mexico
Ctr Med Nacl Siglo XXI, Un Invest Med Bioquim, Inst Mexicano Seguro Social, Mexico City, Mexico
(Corresponding Author), Inst Nacl Salud Publ, Ctr Invest Enfermedades Infecciosas, Cuernavaca, Mexico
gold, All Open Access, Gold
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