Rhizobium tropici and Riboflavin Amendment Condition Arbuscular Mycorrhiza Colonization in Phaseolus vulgaris L.
Por:
Banuelos, Jacob, Martinez-Romero, Esperanza, Montano, Noe Manuel, Camargo-Ricalde, Sara Lucia
Publicada:
1 mar 2023
Categoría:
Agronomy and crop science
Resumen:
Phaseolus vulgaris L. (Fabaceae) forms symbioses with arbuscular
mycorrhizal fungi (AMF) and nitrogen-fixing rhizobia (NFB). The
tripartite relationship uses molecular singals to establish
intracellular symbioses in roots. The goal of this study was to
determine if Rhizobium tropici CIAT 899 and exogenous riboflavin
(vitamin B2) have an effect on AMF species selection and root
colonization of P. vulgaris. Using SSU rRNA fragment amplification of
DNA extracted from P. vulgaris roots, we found that the presence of R.
tropici altered the relative distribution of AMF species. Dominikia
bernensis (Ohel) was the most abundant AMF species in P. vulgaris roots
but when R. tropici was co-inoculated, Glomus species dominated.
Rhizobacteria such as R. tropici, secrete riboflavin and could affect
AMF symbiosis. Addition of 50 mu M riboflavin to P. vulgaris, increased
plant growth (28%), dry nodule weight (18%), AMF colonization (248%)
and mycorrhizal vesicle frequency (56%) in bean roots. 3.12 and 12.5 mu
M riboflavin favored the presence of Glomus macrocarpum in P. vulgaris
roots. This work provides the basis to further study of rhizobial and
mycorrhizal co-inoculation of Phaseolus vulgaris bean.
Filiaciones:
Univ Autonoma Metropolitana, Doctorado Ciencias Biol & Salud, Ave Ferrocarill San Rafael Atlixco 186, Mexico City 09310, Mexico
Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Ave Univ 2001, Cuernavaca 62210, Mexico
Univ Autonoma Metropolitana, Dept Biol, Div Ciencias Biol & Salud, Unidad Iztapalapa, Ave Ferrocarill San Rafael Atlixco 186, Mexico City 09310, Mexico
Green Published, gold, All Open Access, Gold, Green
|