Aerobic training improves bone fragility by reducing the inflammatory microenvironment in bone tissue in type 2 diabetes
Por:
Cifuentes-Mendiola, Saul Ernesto, Solis-Suarez, Diana Laura, Martinez-Davalos, Arnulfo, Perrusquia-Hernandez, Elias, Garcia-Hernandez, Ana Lilia
Publicada:
1 dic 2022
Resumen:
Aerobic training (AT) is indicated in type 2 diabetes mellitus (T2DM) to
control hyperglycaemia and inflam-mation. AT improves bone
microarchitecture and resistance to fracture. The intensity of AT and
the mechanisms that lead to the improvement in bone quality are still
unknown. Using a mouse model of T2DM, we evaluated the effects of two
intensities of forced AT. We divided mice into: sedentary (SED),
T2DM-SED, low runners (LOW), T2DM-LOW, high runners (HIGH) and
T2DM-HIGH. The AT for low was 8 m/minute (m/min); 5 degrees slope or
high 18 m/min; 15 degrees slope for 2 months. We measured metabolic
parameters, the serum cytokines concentration, lipocalin-2 (LCN-2) and
adiponectin; and the tibial concentrations of LCN-2, tumour necrosis
factor alpha (TNF-alpha) and protein carbonylation (CO). We evaluated
femur morphometry and biomechanical properties. We per-formed multiple
correlation analysis. The T2DM-LOW versus T2DM-SED group, shown an
increase of interleukin (IL)-10 (417 +/- 90 vs 102 +/- 25 pg/mL) and
improved trabecular bone (BV/TV: 31.8 +/- 2.3 vs 19.25 +/- 1.4%; Tb.
Sp.: 1.62 +/- 0.02 vs 2.0 +/- 0.07 mm), by a decrease bone CO (3.4 +/-
0.1 vs 6.0 +/- 0.5 nmol/mg), bone TNF-alpha (84 +/- 4 vs 239 +/- 13
pg/mL) and LCN-2 (2887 +/- 23 vs 3418 +/- 105 pg/mL). The T2DM-HIGH
versus T2DM-SED group showed a greater hypoglycaemic effect (228 +/- 10
vs 408 +/- 5 mg/dL), with improved cortical bone density (0.26 +/- 0.012
vs 0.21 +/- 0.007 mm) and fracture resistance (102 +/- 8 vs 78 +/- 5
MPa), by a reduction of bone TNF-alpha (77 +/- 34 vs 239 +/- 13 pg/mL);
LCN-2 (2768 +/- 20 vs 3418 +/- 105 pg/mL) and CO (4.8 +/- 0.5 vs 6.0 +/-
0.5 nmol/mg). In conclusion, AT improves bone morphometry and
biomechanical properties by reducing the bone inflammatory
microenvironment.
Filiaciones:
Laboratory of Dental Research, Section of Osteoimmunology and Oral Immunology. FES Iztacala, National Autonomous University of Mexico (UNAM), Mexico
Postgraduate in Biological Sciences. National Autonomous University of Mexico (UNAM), Mexico City, Mexico
Postgraduate in Dentistry Sciencies. National Autonomous University of Mexico (UNAM), Mexico City, Mexico
Physics Institute, National Autonomous University of Mexico (UNAM), Mexico
Laboratory of Dental Research, Neurobiology of Oral Sensations and Movements Section, FES Iztacala, National Autonomous University of Mexico (UNAM), Mexico
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