Dissection of the autophagic route in oocytes from atretic follicles
Por:
Castro-Cruz, Abraham, Echeverria, Olga M. M., Sanchez-Sanchez, Luis, Munoz-Velasco, Israel, Juarez-Chavero, Silvia, Torres-Ramirez, Nayeli, Vazquez-Nin, Gerardo H. H., Escobar, Maria Luisa
Publicada:
1 mar 2023
Ahead of Print:
1 ene 2023
Resumen:
Background InformationAutophagy is a conserved process that functions as
a cytoprotective mechanism; it may function as a cell death process
called programmed cell death type II. There is considerable evidence for
the presence of autophagic cell death during oocyte elimination in
prepubertal rats. However, the mechanisms involved in this process have
not been deciphered. ResultsOur observations revealed autophagic cell
death in oocytes with increased labeling of the autophagic proteins
Beclin 1, light chain 3 A (LC3 A), and lysosomal-associated membrane
protein 1 (Lamp1). Furthermore, mTOR and phosphorylated (p)-mTOR (S2448)
proteins were significantly decreased in oocytes with increased levels
of autophagic proteins, indicating autophagic activation. Moreover,
phosphorylated protein kinase B (p-AKT) was not expressed by oocytes,
but mitogen-activated protein kinase/extracellular signalregulated
kinase (MAPK/ERK) signaling was observed. Additionally, selective and
elevated mitochondrial degradation was identified in altered oocytes.
ConclusionsAll these results suggest that mTOR downregulation, which
promotes autophagy, could be mediated by low energy levels and sustained
starvation involving the phosphoinositide 3-kinase (PI3K)/AKT/mTOR and
MAPK/ERK pathways. SignificanceIn this work, we analyzed the manner in
which autophagy is carried out in oocytes undergoing autophagic cell
death by studying the behavior of proteins involved in different steps
of the autophagic pathway.
Filiaciones:
Castro-Cruz, Abraham:
Univ Nacl Autonomade Mexico, Col Univ Nacl Autonoma Mex, Dept Biol Celular, Lab Microscopia Elect,Fac Ciencias, Ciudad de Mexico, Mexico
Echeverria, Olga M. M.:
Univ Nacl Autonomade Mexico, Col Univ Nacl Autonoma Mex, Dept Biol Celular, Lab Microscopia Elect,Fac Ciencias, Ciudad de Mexico, Mexico
Sanchez-Sanchez, Luis:
Univ Nacl Autonoma Mexico, Fac Estudios Super Zaragoza, Lab Biol Mol Canc, Lab 6,2 do piso, Mexico City, Mexico
Munoz-Velasco, Israel:
Univ Nacl Autonomade Mexico, Col Univ Nacl Autonoma Mex, Dept Biol Celular, Lab Microscopia Elect,Fac Ciencias, Ciudad de Mexico, Mexico
Juarez-Chavero, Silvia:
Univ Nacl Autonomade Mexico, Col Univ Nacl Autonoma Mex, Dept Biol Celular, Lab Microscopia Elect,Fac Ciencias, Ciudad de Mexico, Mexico
Torres-Ramirez, Nayeli:
Univ Nacl Autonomade Mexico, Col Univ Nacl Autonoma Mex, Dept Biol Celular, Lab Microscopia Elect,Fac Ciencias, Ciudad de Mexico, Mexico
Vazquez-Nin, Gerardo H. H.:
Univ Nacl Autonomade Mexico, Col Univ Nacl Autonoma Mex, Dept Biol Celular, Lab Microscopia Elect,Fac Ciencias, Ciudad de Mexico, Mexico
Escobar, Maria Luisa:
Univ Nacl Autonomade Mexico, Col Univ Nacl Autonoma Mex, Dept Biol Celular, Lab Microscopia Elect,Fac Ciencias, Ciudad de Mexico, Mexico
Univ Nacl Autonoma Mexico, Col Univ Nacl Autonoma Mexico, Fac Ciencias, Dept Biol Celular, Ave Univ 3000, Ciudad de Mexico 04510, Mexico
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