Oxidized glutathione promotes the association of proteins from bean seeds to potato starch
Por:
Correa P., Bernal L., Coello P., Martínez-Barajas E.
Publicada:
1 ene 2012
Categoría:
Chemistry (miscellaneous)
Resumen:
In this paper, we report the use of oxidized glutathione (GSSG) in promoting the association of bean seed proteins to starch granules purified from potato tubers. The resulting interaction was stable and only a small fraction of the bound proteins was released by washing with 5% glucose. Higher concentrations of glucose or other sugars (fructose, mannose and mannitol) failed to further increase protein release. Dithiothreitol (DTT) at concentrations up to 50 mM did not liberate the proteins associated to starch; however, applied after GSSG but before adding the proteins, it was very effective in prevent- ing binding of proteins to starch. Lastly, starch modified by the bound proteins increased its water absorption capacity. © 2012, Sociedad Química de México.
Filiaciones:
Correa P.:
Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, Mexico 04510, D.F., Mexico
Bernal L.:
Facultad de Ciencias Químicas, Universidad La Salle, Mexico
Coello P.:
Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, Mexico 04510, D.F., Mexico
Martínez-Barajas E.:
Departamento de Bioquímica, Facultad de Química, Universidad Nacional Autónoma de México, Ciudad Universitaria, Coyoacán, Mexico 04510, D.F., Mexico
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