Conversion of M1 Macrophages to Foam Cells: Transcriptome Differences Determined by Sex
Por:
Nambo-Venegas, Rafael, Palacios-Gonzalez, Berenice, Mas-Oliva, Jaime, Aurioles-Amozurrutia, Ana Karen, Cruz-Rangel, Armando, Moreno, Abel, Hidalgo-Miranda, Alfredo, Rodriguez-Dorantes, Mauricio, Vadillo-Ortega, Felipe, Xicohtencatl-Cortes, Juan, Ruiz-Olmedo, Maria Isabel, Reyes-Grajeda, Juan Pablo
Publicada:
1 feb 2023
Resumen:
Background: M1 macrophages involved in pro-inflammatory processes can be
induced by low-density lipoproteins (LDL), giving rise to foam cells. In
the atheroma plaque, it has been identified that males present more
advanced lesions associated with infiltration. Therefore, our study aims
to investigate sex-related changes in the transcriptome of M1
macrophages during the internalization process of LDL particles.
Methods: Peripheral blood mononuclear cells (PBMCs) from healthy male
and female subjects were separated using Hystopaque, and monocytes were
isolated from PBMCs using a positive selection of CD14+ cells. Cells
were stimulated with LDL 10 mu g/mL, and the transcriptional profile of
M1 macrophages performed during LDL internalization was determined using
a Clariom D platform array. Results: Chromosome Y influences the immune
system and inflammatory responses in males expressing 43% of
transcripts in response to LDL treatment. Males and females share 15
transcripts, where most correspond to non-coding elements involved in
oxidative stress and endothelial damage. Conclusions: During LDL
internalization, male monocyte-derived M1 macrophages display more
marked proinflammatory gene expression. In contrast, female M1
macrophages display a more significant number of markers associated with
cell damage.
Filiaciones:
Nambo-Venegas, Rafael:
Laboratorio de Estructura de Proteínas, Instituto Nacional de Medicina Genómica, Mexico City, 14600, Mexico
Palacios-Gonzalez, Berenice:
Laboratorio de Envejecimiento Saludable, Centro de Investigación Sobre Envejecimiento (CIE-CINVESTAV Sur), Instituto Nacional de Medicina Genómica, Mexico City, 14330, Mexico
Mas-Oliva, Jaime:
Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, 04510, Mexico
Aurioles-Amozurrutia, Ana Karen:
Laboratorio de Estructura de Proteínas, Instituto Nacional de Medicina Genómica, Mexico City, 14600, Mexico
Cruz-Rangel, Armando:
Laboratorio de Estructura de Proteínas, Instituto Nacional de Medicina Genómica, Mexico City, 14600, Mexico
Moreno, Abel:
Instituto de Química, Universidad Nacional Autónoma de México, Mexico City, 04510, Mexico
Hidalgo-Miranda, Alfredo:
Laboratorio de Genómica del Cáncer, Instituto Nacional de Medicina Genómica, Mexico City, 14600, Mexico
Rodriguez-Dorantes, Mauricio:
Laboratorio de Oncogenomica, Instituto Nacional de Medicina Genómica, Mexico City, 14600, Mexico
Vadillo-Ortega, Felipe:
Unidad de Vinculación Científica de la Facultad de Medicina UNAM en INMEGEN, Instituto Nacional de Medicina Genómica, Mexico City, 14600, Mexico
Xicohtencatl-Cortes, Juan:
Laboratorio de Bacteriología Intestinal, Hospital Infantil de México Federico Gómez, Mexico City, 06720, Mexico
Ruiz-Olmedo, Maria Isabel:
ROPPEN RT de R.L. de C.V., Mexico City, 14650, Mexico
Reyes-Grajeda, Juan Pablo:
Laboratorio de Estructura de Proteínas, Instituto Nacional de Medicina Genómica, Mexico City, 14600, Mexico
gold, All Open Access; Gold
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