Biofouling and biocorrosion by microbiota from a marine oil pipeline: A metagenomic and proteomic approach
Por:
Avelino-Jimenez, I. A., Hernandez-Maya, L., Larios-Serrato, V., Quej-Ake, L., Castelan-Sanchez, H., Herrera-Diaz, J., Garibay-Febles, V., Rivera-Olvera, J. N., Zavala-Olivares, G., Zapata-Penasco, I.
Publicada:
1 abr 2023
Ahead of Print:
1 feb 2023
Resumen:
Biofouling and biocorrosion in oil pipelines are serious problems
capable of causing expensive, irreparable losses. For a better
understanding of biocorrosion processes, several lines of evidence are
required, not least experi-mental considerations that emulate natural
conditions inside pipelines. Herein, an omics approach is used to unveil
the microbial involvement in the deterioration and electrochemical
process on X52 steel with Shewanella (SW), the oil pipeline microbiota
(AK), and a mixed culture (AK5), the latter two obtained from a sludge
of pig pipeline cleaning in the Gulf of Mexico. Shotgun metagenomics
showed diverse communities at species level in pipeline sludge: bacteria
up to 112, 34 archaea, 9 fungi, and 23 viruses. In AK the most abundant
taxa were Petrotogaceae with Petrotoga (31.16%) and Methanomicrobiaceae
(48.51%). In AK5 were Methylobacterium (49%) and Methanomicrobiaceae
(19.49%). Concerning fungi and viruses, respectively: Clavariaceae
(43.26%), Picciniaceae (35.59), Onygenaceae (3.77%), Siphoviridae
(46.8%), Myoviridae (33.50%), Podoviridae (7.36%) in AK5. Proteomics
revealed 32,284 proteins in AK, 13,664 in AK5, and 7 in SW. Expressed
proteins were oxido-reductases, lyases, nitrogenases, metalloproteases,
and hydrolases associated with the biocorrosion process, for instance,
electron transport, sulfur oxidation, nitrate reduction, biofilm
formation, and oxygen reduction. In addition to the functional
annotation of metagenomes in KEGG pathways, through the Multigenomic
Entropy Based Score (MEBS) the metabolic functions were evaluated
explaining the relation to energy transfer processes of the
biogeochemical cycles. The sludge was analyzed by X-ray diffraction
(XRD), X-ray fluorescence (XRF), and Mo center dot ssbauer spectroscopy
suggesting the microbiota involvement in the biofouling and biocorrosion
processes. Regarding the electrochemical assessments, scanning electron
microscopy (SEM) and Energy Dispersive X-ray Spectroscopy (EDX) coupled
to SEM, AK, AK5, and SW biofilms on X52 steel behaved differently. Three
elec-trochemical mechanisms of biocorrosion are proposed.
Filiaciones:
Instituto Mexicano Del Petróleo, Eje Central Lázaro Cárdenas, Ciudad de México, 07730, Mexico
Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. Carpio y Plan de Ayala, Ciudad de México, 11340, Mexico
Programa de Investigadores Por México, Consejo Nacional de Ciencia y Tecnología, Av. Insurgentes Sur 1582, Crédito Constructor, Benito Juárez, Ciudad de México, 03940, Mexico
Facultad de Química, Universidad Nacional Autónoma de México, Circuito Exterior S/N, Coyoacán, Cd. Universitaria, Ciudad de México, 04510, Mexico
Tecnológico Nacional de México, Estado de México, Ixtapaluca, 56580, Mexico
hybrid, All Open Access; Hybrid Gold
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