Protein Profiling of Psittacanthus calyculatus during Mesquite Infection
Por:
Aguilar-Venegas, Montserrat, Quintana-Rodriguez, Elizabeth, Aguilar-Hernandez, Victor, Lopez-Garcia, Claudia Marina, Conejo-Davila, Efrain, Brito-Argaez, Ligia, Loyola-Vargas, Victor M., Vega-Arreguin, Julio, Orona-Tamayo, Domancar
Publicada:
1 feb 2023
Resumen:
Psittacanthus calyculatus is a hemiparasite mistletoe that represents an
ecological problem due to the impacts caused to various tree species of
ecological and commercial interest. Although the life cycle for the
Psittacanthus genus is well established in the literature, the
development stages and molecular mechanism implicated in P. calyculatus
host infection are poorly understood. In this study, we used a
manageable infestation of P. laevigata with P. calyculatus to clearly
trace the infection, which allowed us to describe five phenological
infective stages of mistletoe on host tree branches: mature seed (T1),
holdfast formation (T2), haustorium activation (T3), haustorium
penetration (T4), and haustorium connection (T5) with the host tree.
Proteomic analyses revealed proteins with a different accumulation and
cellular processes in infective stages. Activities of the cell
wall-degrading enzymes cellulase and beta-1,4-glucosidase were primarily
active in haustorium development (T3), while xylanase, endo-glucanase,
and peptidase were highly active in the haustorium penetration (T4) and
xylem connection (T5). Patterns of auxins and cytokinin showed spatial
concentrations in infective stages and moreover were involved in
haustorium development. These results are the first evidence of
proteins, cell wall-degrading enzymes, and phytohormones that are
involved in early infection for the Psittacanthus genus, and thus
represent a general infection mechanism for other mistletoe species.
These results could help to understand the molecular dialogue in the
establishment of P. calyculatus parasitism.
Filiaciones:
Aguilar-Venegas, Montserrat:
Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores, Unidad León, UNAM, Guanajuato, León, 37684, Mexico
Quintana-Rodriguez, Elizabeth:
Medio Ambiente y Biotecnología, CIATEC, A.C, Guanajuato, León, 37545, Mexico
Aguilar-Hernandez, Victor:
Unidad de Bioquímica y Biología Molecular de Plantas, CICY, A.C, Yucatán, Mérida, CP 97205, Mexico
Lopez-Garcia, Claudia Marina:
Medio Ambiente y Biotecnología, CIATEC, A.C, Guanajuato, León, 37545, Mexico
Conejo-Davila, Efrain:
Instituto Politécnico Nacional, Unidad Profesional Interdisciplinaria de Ingeniería Campus Guanajuato, Guanajuato, Silao de la Victoria, CP 36275, Mexico
Brito-Argaez, Ligia:
Unidad de Bioquímica y Biología Molecular de Plantas, CICY, A.C, Yucatán, Mérida, CP 97205, Mexico
Loyola-Vargas, Victor M.:
Unidad de Bioquímica y Biología Molecular de Plantas, CICY, A.C, Yucatán, Mérida, CP 97205, Mexico
Vega-Arreguin, Julio:
Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores, Unidad León, UNAM, Guanajuato, León, 37684, Mexico
Orona-Tamayo, Domancar:
Medio Ambiente y Biotecnología, CIATEC, A.C, Guanajuato, León, 37545, Mexico
gold, Green Published, All Open Access, Gold, Green
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