Omics Analyses of Trichomonas vaginalis Actin and Tubulin and Their Participation in Intercellular Interactions and Cytokinesis
Por:
Lorenzo-Benito S., Rivera-Rivas L.A., Sánchez-Ayala L., Ortega-López J., Montes-Flores O., Talamás-Lara D., Arroyo R.
Publicada:
1 ene 2022
Resumen:
Actin and tubulin proteins from Trichomonas vaginalis are crucial for morphogenesis and mitosis. This parasite has 10 and 11 genes coding bonafide actin and tubulin proteins, respectively. Hence, the goal of this work was to analyze these actin and tubulin genes, their expression at the mRNA and protein levels, and their parasite localization in intercellular interaction and cytokinesis. Representative bonafide actin (tvact1) and tubulin (tvtuba1) genes were cloned into and expressed in Escherichia coli. The recombinant proteins TvACT1r and TvTUBa1r were affinity purified and used as antigens to produce polyclonal antibodies. These antibodies were used in 1DE and 2DE WB and indirect immunofluorescence assays (IFA). By IFA, actin was detected as a ring on the periphery of ameboid, ovoid, and cold-induced cyst-like parasites, on pseudopods of amoeboid parasites, and in cytoplasmic extensions (filopodia) in cell–cell interactions. Tubulin was detected in the axostyle, flagellum, undulating membrane, and paradesmose during mitosis. Paradesmose was observed by IFA mainly during cytokinesis. By scanning electron microscopy, a tubulin-containing nanotubular structure similar to the tunneling nanotubes (TNTs) was also detected in the last stage of cytokinesis. In conclusion, actin and tubulin are multigene families differentially expressed that play important roles in intercellular interactions and cytokinesis. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
Filiaciones:
Lorenzo-Benito S.:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN #2508, Col. San Pedro Zacatenco, Alcaldía Gustavo A. Madero, Mexico City, CP 07360, Mexico
Rivera-Rivas L.A.:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN #2508, Col. San Pedro Zacatenco, Alcaldía Gustavo A. Madero, Mexico City, CP 07360, Mexico
Sánchez-Ayala L.:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN #2508, Col. San Pedro Zacatenco, Alcaldía Gustavo A. Madero, Mexico City, CP 07360, Mexico
Ortega-López J.:
Departamento de Biotecnología y Bioingeniería, CINVESTAV-IPN, Av. IPN #2508, Col. San Pedro Zacatenco, Alcaldía Gustavo A. Madero, Mexico City, CP 07360, Mexico
Montes-Flores O.:
Departamento de Biotecnología y Bioingeniería, CINVESTAV-IPN, Av. IPN #2508, Col. San Pedro Zacatenco, Alcaldía Gustavo A. Madero, Mexico City, CP 07360, Mexico
Talamás-Lara D.:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN #2508, Col. San Pedro Zacatenco, Alcaldía Gustavo A. Madero, Mexico City, CP 07360, Mexico
Arroyo R.:
Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional (CINVESTAV-IPN), Av. IPN #2508, Col. San Pedro Zacatenco, Alcaldía Gustavo A. Madero, Mexico City, CP 07360, Mexico
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