Ice plant root plasma membrane aquaporins are regulated by clathrin-coated vesicles in response to salt stress
Por:
Gómez-Méndez M.F., Amezcua-Romero J.C., Rosas-Santiago P., Hernández-Domínguez E.E., de Luna-Valdez L.A., Ruiz-Salas J.L., Vera-Estrella R., Pantoja O.
Publicada:
1 ene 2023
Resumen:
The regulation of root Plasma membrane (PM) Intrinsic Protein (PIP)-type aquaporins (AQPs) is potentially important for salinity tolerance. However, the molecular and cellular details underlying this process in halophytes remain unclear. Using free-flow electrophoresis and label-free proteomics, we report that the increased abundance of PIPs at the PM of the halophyte ice plant (Mesembryanthemum crystallinum L.) roots under salinity conditions is regulated by clathrin-coated vesicles (CCV). To understand this regulation, we analyzed several components of the M. crystallinum CCV complexes: clathrin light chain (McCLC) and subunits µ1 and µ2 of the adaptor protein (AP) complex (McAP1µ and McAP2µ). Co-localization analyses revealed the association between McPIP1;4 and McAP2µ and between McPIP2;1 and McAP1µ, observations corroborated by mbSUS assays, suggesting that AQP abundance at the PM is under the control of CCV. The ability of McPIP1;4 and McPIP2;1 to form homo- and hetero-oligomers was tested and confirmed, as well as their activity as water channels. Also, we found increased phosphorylation of McPIP2;1 only at the PM in response to salt stress. Our results indicate root PIPs from halophytes might be regulated through CCV trafficking and phosphorylation, impacting their localization, transport activity, and abundance under salinity conditions. © American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com.
Filiaciones:
Gómez-Méndez M.F.:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
Amezcua-Romero J.C.:
Departamento de Ciencias Agrogenómicas, Escuela Nacional de Estudios Superiores, Unidad León, Universidad Nacional Autónoma de MéxicoLeón, Mexico
Rosas-Santiago P.:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
Hernández-Domínguez E.E.:
Catedrático CONACyT en la Red de Estudios Moleculares Avanzados, Instituto de Ecología A.C., Mexico
de Luna-Valdez L.A.:
Department of Microbiology & Plant Pathology, Institute for Integrative Genome Biology, University of California, Riverside, CA, United States
Ruiz-Salas J.L.:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
Vera-Estrella R.:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
Pantoja O.:
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico
|