A Novel Microcystin-Producing Cyanobacterial Species from the Genus Desmonostoc, Desmonostoc alborizicum sp. nov., Isolated from a Water Supply System of Iran
Por:
Nowruzi, Bahareh, Becerra-Absalon, Itzel, Metcalf, James S.
Publicada:
1 ene 2023
Resumen:
A qanat or kariz is a slightly sloping underground aqueduct used to
transport water from wells or aquifers to the surface for irrigation and
drinking supply. A cyanobacterial strain was isolated from a
cyanobacterial mat colonizing the wall of a qanat in Golestan province,
Gorgan City, Iran. Fragments of 16S rRNA, mcyG, and mcyD genes were
amplified and sequenced, as well as the 16S-23S internal transcribed
spacer (ITS). After microscopic examination, the isolate was related to
a morphotype of Nostoc sensu lato group, with similar characteristics to
Desmonostoc. The 16S rRNA phylogenetic analysis placed the isolate into
the typical cluster of the recently proposed genus Desmonostoc.
Morphological analysis revealed distinctive characteristic and secondary
16S-23S rRNA structures derived from comparative analysis, which did not
match known species of Desmonostoc. These results lead us to propose a
novel Desmonostoc species, Desmonostoc alborizicum, which was described
and compared with similar taxa. Furthermore, for the first time a
potentially toxic species of Desmonostoc was isolated from a water
supply, since the mcyD and mcyG genes of the microcystin synthetase
(mcy) cluster were successfully sequenced. Using mass spectrometry,
detectable amounts of the hepatotoxin microcystin-LR and -RR, along with
demethylated variants, were present in cell extracts of the Desmonostoc
strain. Our findings contribute to a deeper understanding of the
diversity, systematics, and occurrence of the genus Desmonostoc.
Filiaciones:
Nowruzi, Bahareh:
Department of Biotechnology, Science and Research Branch, Islamic Azad University, Daneshgah Blvd, Simon Bulivar Blvd, Tehran, Iran
Becerra-Absalon, Itzel:
Laboratorio de Ficología, Facultad de Ciencias, Universidad Nacional Autónoma de México, Ciudad de Mexico, Mexico
Metcalf, James S.:
Department of Biological Sciences, Bowling Green State University, Bowling Green, OH 43403, United States
Brain Chemistry Labs, Box 3464, Jackson, WY 83001, United States
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