Extracellular matrix hydrogel derived from bovine bone is biocompatible in vitro and in vivo
Por:
Ayala-Ham, Alfredo, Aguilar-Medina, Maribel, Leon-Felix, Josefina, Geovanni Romero-Quintana, Jose, Bermudez, Mercedes, Lopez-Gutierrez, Jorge, Jimenez-Gastelum, German, Avendano-Felix, Mariana, Lizarraga-Verdugo, Erik, Castillo-Ureta, Hipolito, Lopez-Camarillo, Cesar, Ramos-Payan, Rosalio
Publicada:
1 ene 2022
Resumen:
BACKGROUND: Nowadays, biomaterials used as a scaffold must be easy to
deliver in the bone defect area. Extracellular matrix (ECM) hydrogels
are highly hydrated polymers that can fill irregular shapes and act as
bioactive materials.
OBJECTIVE: This work aims to show the effects of ECM hydrogels derived
from bovine bone (bECMh) on proliferation, cytotoxicity and expression
of pro-inflammatory cytokines in three cells types involved in tissue
regeneration, as well as biocompatibility in vivo.
METHODS: In vitro, we used an extract of bECMh to test it on
macrophages, fibroblasts, and adipose-derived mesenchymal stem cells
(AD-MCSs). Cell proliferation was measured using the MTT assay,
cytotoxicity was measured by quantifying lactate dehydrogenase release
and the Live/Dead Cell Imaging assays. Concentrations of IL-6, IL-10,
IL-12p70, MCP-1 and TNF-alpha were quantified in the supernatants using
a microsphere-based cytometric bead array. For in vivo analysis, Wistar
rats were inoculated into the dorsal sub-dermis with bECMh, taking as
reference the midline of the back. The specimens were sacrificed at 24 h
for histological study.
RESULTS: In vitro, this hydrogel behaves as a dynamic biomaterial that
increases fibroblast proliferation, induces the production of
pro-inflammatory cytokines in macrophages, among which MCP-1 and TNF-a
stand out. In vivo, bECMh allows the colonization of host
fibroblast-like and polymorphonuclear cells, without tissue damage or
inflammation.
CONCLUSIONS: The results indicate that bECMh is a biocompatible material
that could be used as a scaffold, alone or in conjunction with cells or
functional biomolecules, enhancing proliferation and allowing the
filling of bone defects to its further regeneration.
Filiaciones:
Ayala-Ham, Alfredo:
Autonomous Univ Sinaloa, Fac Biol, Culiacan, Sinaloa, Mexico
Autonomous Univ Sinaloa, Fac Odontol, Culiacan, Sinaloa, Mexico
Aguilar-Medina, Maribel:
Autonomous Univ Sinaloa, Fac Biol & Chem Sci, Culiacan, Sinaloa, Mexico
Leon-Felix, Josefina:
Ctr Res Food & Dev CIAD, Culiacan, Sinaloa, Mexico
Geovanni Romero-Quintana, Jose:
Autonomous Univ Sinaloa, Fac Biol & Chem Sci, Culiacan, Sinaloa, Mexico
Bermudez, Mercedes:
Autonomous Univ Chihuahua, Fac Odontol, Chihuahua, Mexico
Lopez-Gutierrez, Jorge:
Autonomous Univ Sinaloa, Fac Biol, Culiacan, Sinaloa, Mexico
Jimenez-Gastelum, German:
Autonomous Univ Sinaloa, Fac Biol, Culiacan, Sinaloa, Mexico
Avendano-Felix, Mariana:
Autonomous Univ Sinaloa, Fac Biol & Chem Sci, Culiacan, Sinaloa, Mexico
Lizarraga-Verdugo, Erik:
Autonomous Univ Sinaloa, Fac Biol & Chem Sci, Culiacan, Sinaloa, Mexico
Castillo-Ureta, Hipolito:
Autonomous Univ Sinaloa, Fac Biol, Culiacan, Sinaloa, Mexico
Lopez-Camarillo, Cesar:
Autonomous Univ Mexico City, Mexico City, DF, Mexico
Ramos-Payan, Rosalio:
Autonomous Univ Sinaloa, Fac Biol & Chem Sci, Culiacan, Sinaloa, Mexico
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