Identification of Antifungal Compounds from Avocado Rhizobacteria (Bacillus spp.) against Fusarium spp., by a Bioassay-Guided Fractionation Approach
Por:
Guevara-Avendano, Edgar, Lizette Perez-Molina, Mayra, Luis Monribot-Villanueva, Juan, Marian Cortazar-Murillo, Elvis, Ramirez-Vazquez, Monica, Reverchon, Frederique, Antonio Guerrero-Analco, Jose
Publicada:
1 nov 2022
Ahead of Print:
1 oct 2022
Resumen:
Antimicrobial compounds produced by bacteria have been increasingly
acknowledged as an important resource for the control of phytopathogens.
We used a bioassay-guided fractionation approach to identify antifungal
metabolites produced by two avocado rhizobacteria (INECOL-4742 and
INECOL-5927), both members of the Bacillus subtilis/B. amyloliquefaciens
species complex, against Fusarium solani and F. kuroshium, causal agent
of Fusarium dieback in avocado and other hosts. The butanol (BuOH)
organic extract from INECOL-4742 (B1-Bu) exhibited the highest
percentage of inhibition (PI) against F. solani (78.76 %), also
inhibiting F. kuroshium by up to 44.30 %. Primary fractions, Bu-F3,
Bu-F12 and Bu-F15, obtained by silica gel open column chromatography,
exhibited the highest PI against F. solani (28.57 % to 33.50 %) and F.
kuroshium (38.78 % to 45.00 %). The presence of cyclic lipopeptides
from the iturin, surfactin and fengycin families in B1-Bu extracts and
primary fractions was determined by UPLC-ESI-HRMS. The Confocal Laser
Microscopy analysis revealed deformations in the hyphae of F. kuroshium
exposed to extracts, primary fractions and C-13 surfactin chemical
standard. These results emphasize the potential of natural products from
Bacillus for the control of the emerging phytopathogenic fungus F.
kuroshium.
Filiaciones:
Guevara-Avendano, Edgar:
Inst Ecol, Ctr Reg Bajio, Lab Microbiol Ambiental Red Estudios Mol Avanzado, Patzcuaro 61600, Michoacan, Mexico
Lizette Perez-Molina, Mayra:
Inst Ecol, Lab Quim Prod Nat Red Estudios Mol Avanzados, Xalapa 91073, Veracruz, Mexico
Luis Monribot-Villanueva, Juan:
Inst Ecol, Lab Quim Prod Nat Red Estudios Mol Avanzados, Xalapa 91073, Veracruz, Mexico
Marian Cortazar-Murillo, Elvis:
Inst Ecol, Lab Quim Prod Nat Red Estudios Mol Avanzados, Xalapa 91073, Veracruz, Mexico
Ramirez-Vazquez, Monica:
Univ Nacl Autonoma Mexico, Fac Ciencias, Cdmx 04510, Mexico
Reverchon, Frederique:
Inst Ecol, Ctr Reg Bajio, Lab Microbiol Ambiental Red Estudios Mol Avanzado, Patzcuaro 61600, Michoacan, Mexico
Antonio Guerrero-Analco, Jose:
Inst Ecol, Lab Quim Prod Nat Red Estudios Mol Avanzados, Xalapa 91073, Veracruz, Mexico
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