Rapid Communication: Evaluation of methane inhibitor 3-nitrooxypropanol and monensin in a high-grain diet using the rumen simulation technique (Rusitec)
Por:
Romero-Pérez A., Okine E.K., Guan L.L., Duval S.M., Kindermann M., Beauchemin K.A.
Publicada:
1 ene 2017
Resumen:
The objective of this study was to evaluate the effects of 3-nitrooxypropanol (NOP), a known methane (CH4) inhibitor; the ionophore monensin (MON); and their combination on in vitro CH4 production in a high-grain diet (85% barley grain, 10% barley silage, and 5% vitamin-mineral supplement; DM basis) using a rumen simulation technique (Rusitec). Sixteen fermentation vessels in 2 Rusitec apparatuses (blocks) were used in a completely randomized block design with 4 treatments: Control, NOP (200 µg/g DM), MON (200 µg/g DM), and the combination of 200 µg NOP/g DM and 200 µg MON/g DM (NOP + MON). Two fermenters within each apparatus were randomly assigned to a treatment. Treatments were mixed with 10 g of substrate and supplied on a daily basis. The study included an 8-d adaptation period without treatment supplementation and a 6-d period for addition of treatments. Dry matter disappearance, pH, and total VFA were not affected by treatment (P = 0.34). Acetate proportion was decreased by 8.3% and 14.9% with NOP and NOP + MON (P < 0.01), respectively; however, propionate proportion was not affected by treatment (P = 0.44). The acetate to propionate ratio was lowered by 21.1% with the combination of NOP and MON (P = 0.02), whereas ammonia-N concentration was not affected by treatment (P = 0.50). Total gas production was unaffected (P = 0.50), but CH4 production decreased by 77.7% and 75.95% (P < 0.01) with NOP and NOP + MON addition, respectively. Concurrently, H2 gas production increased by 131.3% and 185.6% (P = 0.01) with NOP and NOP + MON treatments, respectively. The copy number of methanogens was decreased in both solid and liquid phases (P < 0.01) with NOP and NOP + MON treatments. Despite the combination of NOP + MON showing the greatest decrease in acetate molar proportion and acetate to propionate ratio, it did not further inhibit CH4 beyond the effect of NOP alone. The decrease in CH4 emissions with treatments that included NOP occurred along with a decrease in the copy number of methanogens associated with the solid and liquid phases, confirming the inhibitory effects of NOP on these microorganisms. In conclusion, the combined effects of NOP and MON on CH4 mitigation did not exceed the effect of NOP alone when using a high-grain diet in vitro. © 2017 American Society of Animal Science. All rights reserved.
Filiaciones:
Lethbridge Research and Development Centre, Agriculture and Agri-Food Canada, Lethbridge, AB T1J 4B1, Canada
Department of Biological Sciences, University of Lethbridge, Lethbridge, AB T1K 3M4, Canada
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, AB T6G 2P5, Canada
DSM Nutritional Products France, Research Center for Animal Nutrition, BP 170, Saint Louis Cedex, F-68305, France
DSM Nutritional Products, Basel, CH-4002, Switzerland
All Open Access, Bronze
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